Microarray Technology

This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs, proteins, glycans and whole cells. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microarray Technology: Methods and Applications aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Overview of Microarray Technology.- Surface Functionalization for Immobilization of Probes on Microarrays.- The clickable Method for Oligonucleotide Immobilization onto Azide-Functionalized Microarrays.- Microarray Developed on Plastic Substrates.- Detection and Quantification of Micrornas By Ligase-Assisted Sandwich Hybridization on a Microarray.- Probe Design Strategies for Oligonucleotide Microarrays.- Analyzing Illumina Gene Expression Microarray Data Obtained from Human Whole Blood Cell and Blood Monocyte Samples.- Microarray Technology and its Applications for Detecting Plasma microRNA Biomarkers in Digestive Tract Cancers.- Application of DNA Microarray to Clinical Diagnostics.- High-throughput DNA Array for SNP Detection of KRAS Gene using a Centrifugal Microfluidic Device.- Single Nucleotide Polymorphisms as Genomic Markers for High-Throughput Pharmacogenomic Studies.- DNA Microarray-Based Diagnostics.- High-throughput Screening of Substrate Specificity for Protein Tyrosine Phosphatases (PTPs) On Phosphopeptide Microarrays.- Nanotechnology in the Fabrication of Protein Microarrays.- Epitope Mapping using Peptide Microarray in Autoantibody Profiling.- Preparation of Glycan Arrays using Pyridylaminated Glycans.- Competitive Immunoassays Using Antigen Microarrays.- Parallel Syntheses of Peptides on Teflon-patterned paper arrays (SyntArrays).- Cell Microarrays for Biomedical Applications.