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Microarray Technology

  • Kartonierter Einband
  • 308 Seiten
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This volume provides updates of this established field in both methods and applications, as well as advances in applications of th... Weiterlesen
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This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs, proteins, glycans and whole cells. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microarray Technology: Methods and Applications aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts


Part I: Introduction

1. Overview of Microarray Technology

Paul C. H. Li

Part II: Advances on Techniques in Microarray Technology

2. Surface Functionalization for Immobilization of Probes on Microarrays

C. Desmet and C.A. Marquette

3. The clickable Method for Oligonucleotide Immobilization onto Azide-Functionalized Microarrays

Tomasz Ratajczak, Barbara Uszczyska, Emilia Frydrych-Tomczak, and Marcin K. Chmielewski

4. Microarray Developed on Plastic Substrates

María-José Bañuls; Sergi B. Morais, Luis A. Tortajada-Genaro, and Ángel Maquieira

5. Detection and Quantification of Micrornas by Ligase-Assisted Sandwich Hybridization on a Microarray

Ryo Iizuka, Taro Ueno, and Takashi Funatsu

6. Probe Design Strategies for Oligonucleotide Microarrays

Nicolas Parisot, Eric Peyretaillade, Eric Dugat-Bony, Jérémie Denonfoux, Antoine Mahul, and Pierre Peyret

PART III: Applications of Microarray Technology to Nucleic Acid Assays

7. Analyzing Illumina Gene Expression Microarray Data Obtained from Human Whole Blood Cell and Blood Monocyte Samples

Alexander Teumer, Claudia Schurmann, Arne Schillert, Katharina Schramm, Andreas Ziegler, and Holger Prokisch

8. Microarray Technology and its Applications for Detecting Plasma microRNA Biomarkers in Digestive Tract Cancers

Hirotaka Konishi, Daisuke Ichikawa, Tomohiro Arita, and Eigo Otsuji

9. Application of DNA Microarray to Clinical Diagnostics

Ankita Patel and Sau W. Cheung

10. High-throughput DNA Array for SNP Detection of KRAS Gene using a Centrifugal Microfluidic Device

Abootaleb Sedighi and Paul C. H. Li

11. Single Nucleotide Polymorphisms as Genomic Markers for High-Throughput Pharmacogenomic Studies

Annalisa Lonetti, Maria Chiara Fontana, Giovanni Martinelli, and Ilaria Iacobucci

12. DNA Microarray-Based Diagnostics

Mahsa Gharibi Marzancola, Abootaleb Sedighi, and Paul C. H. Li

Part IV: Applications of Microarray Technology to Other Biological Assays

13. High-throughput Screening of Substrate Specificity for Protein Tyrosine Phosphatases (PTPs) On Phosphopeptide Microarrays

Liqian Gao, Su Seong Lee, Jun Chen, Hongyan Sun, Yuliang Zhao, Zhifang Chai, and Yi Hu

14. Nanotechnology in the Fabrication of Protein Microarrays

Manuel Fuentes, Paula Díez, and Juan Casado-Vela

15. Epitope Mapping using Peptide Microarray in Autoantibody Profiling

Sebastian Henkel, Robert Wellhausen, Dirk Woitalla, Katrin Marcus, and Caroline May

16. Preparation of Glycan Arrays using Pyridylaminated Glycans

Shin-ichi Nakakita and Jun Hirabayashi

17. Competitive Immunoassays Using Antigen Microarrays

Zhaowei Zhang, Weihua Hu, Qi Zhang, Peiwu Li, and Changming Li

18. Parallel Syntheses of Peptides on Teflon-patterned paper arrays (SyntArrays)

Frédérique Deiss, Yang Yang, and Ratmir Derda

19. Cell Microarrays for Biomedical Applications

Mario Rothbauer, David Wartmann, Verena Charwat, and Peter Ertl


Titel: Microarray Technology
Untertitel: Methods and Applications
EAN: 9781493949076
ISBN: 1493949071
Format: Kartonierter Einband
Herausgeber: Springer New York
Genre: Medizin
Anzahl Seiten: 308
Gewicht: 583g
Größe: H254mm x B178mm x T16mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2016

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