This volume provides an overview of advanced protocols for vascular biology research, comprising cardiovascular and lymphatic biology. Chapters detail techniques for isolating and characterizing endothelial and mural cell populations, disseminating signaling pathways and protein-protein interactions, constructing organ-on-chip systems, and using animal models and techniques with bench-to-bedside potential. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Methods in Cardiovascular and Lymphatic Biology aims to provide protocols and applications that can be used by both new investigators and experienced professionals to advance cardiovascular and lymphatic research.

Contains key implementation advice from the experts Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results

Inhalt

Isolation and purification of murine lung endothelial cells.- Two-step Positive Selection Method for the Magnetic Isolation of Lymphatic Endothelial Cells.- Isolation and Enumeration of Circulating Endothelial Cells with Antibody Labeled Staggered Herringbone Microfluidic System.- A Guided Approach to Establish a Functional Humanized Brain-on-Chip Microfluidic Model of the Neurovascular System.- Human iPSC-derived astrocyte-endothelial cell and neuron-endothelial cell co-culture models of the blood-brain barrier to study the impact of ischemic stroke in vitro.- Isolation of rodent aortic smooth muscle cells by enzymatic digestions.- Studying the vascular tone of mouse aortic rings in a standard wire myograph.- Unveiling Central Nervous System Pericytes: Immunofluorescence Protocol and Confocal Microscopy.- Serum-free Mouse Aortic Ring Assay of Angiogenesis.- A New Method to Visualize Retinal Deep Vascular Layers Using Wholemount Tissue Preparations.- Live calcium imaging on mouse primary brain endothelial cells.- Studying nitric oxide in endothelial cells.- Identifying protein-protein interaction or protein phosphorylation by Proximity Ligation Assay (PLA) in endothelial cells.- Label-free imaging with automated tracking and cell-by-cell analysis of migration, morphology, and differentiation of endothelial cells.- Assessment of angiogenic properties of malignant pleural effusion (MPE) fluid.- Ultrasound Contrast Agents in Mice: Bridging the Gap from Preclinical Research to Clinical Translation.