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Mammalian Chromosome Engineering

  • Fester Einband
  • 257 Seiten
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The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with t... Weiterlesen
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The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry. Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.

Provides a precious resource for scientists interested in new ideas in gene technology

Features practical protocols from the cutting-edge of this fast-paced field

Includes expert tips and key implementation advice crucial for getting optimal results

Includes supplementary material: sn.pub/extras

1. Developing Extrachromosomal Gene Expression Vector Technologies: An Overview Richard Wade-Martins 2. High Capacity Extrachromosomal Gene Expression Vectors Olivia Hibbitt and Richard Wade-Martins 3. Naturally Occurring Minichromosome Platforms in Chromosome Engineering: An Overview Elena Raimondi 4. Chromosome Transfer Via Cell Fusion Marianna Paulis 5. Insertional Engineering of Chromosomes with Sleeping Beauty Transposition: An Overview Ivana Grabundzija, Zsuzsanna Izsvák, and Zoltán Ivics 6. Rodent Transgenesis Mediated by a Novel Hyperactive Sleeping Beauty Transposon System Lajos Mátés 7. Construction and Use of a Bottom-Up HAC Vector for Transgene Expression Masashi Ikeno and Nobutaka Suzuki 8. De novo Generation of Satellite DNA-Based Artificial Chromosomes by Induced Large-Scale Amplification Erika Csonka 9. Downstream Bioengineering of ACE Chromosomes for Incorporation of Site-Specific Recombination Cassettes Amy L. Greene and Edward L. Perkins 10. Chromosome Engineering with Lambda-Integrase Mediated Recombination System: The ACE System Tünde Praznovszky 11. Dendrimer Mediated Transfer of Engineered Chromosomes Robert L. Katona 12. Engineered Chromosomes in Transgenics Peter Blazso, Ildiko Sinko, and Robert L. Katona 13. Transfer of Stem Cells Carrying Engineered Chromosomes with XY Clone Laser System Ildiko Sinko and Robert L. Katona 14. Mammalian Artificial Chromosomes and Clinical Applications for Genetic Modification of Stem Cells: An Overview Robert L. Katona, Sandra L. Vanderbyl, and Carl F.Perez 15. Engineered Mammalian Chromosomes in Cellular Protein Production: Future Prospects Malcolm L. Kennard


Titel: Mammalian Chromosome Engineering
Untertitel: Methods and Protocols
EAN: 9781617790980
ISBN: 1617790982
Format: Fester Einband
Herausgeber: Springer-Verlag GmbH
Genre: Medizin
Veröffentlichung: 01.08.2011
Anzahl Seiten: 257
Gewicht: 707g
Größe: H260mm x B183mm x T19mm
Jahr: 2011
Untertitel: Englisch
Auflage: 2011

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