The generation of transgenic mammals, mostly rodents, has become one of the most important approaches in biological and biomedical research, and is of utmost importance in the generation of genetically improved livestock. Edited by respected scholars at the California Institute of Technology, this book provides step-by-step protocols of this important technique. The manual covers not only mice and other rodents, it also describes methods successfully used in cattle, sheep and pigs, making it extremely useful for anyone working on mammalian transgenesis.

Detailed step-by-step protocols, allowing immediate application in the lab Appealing to geneticists in academic and industrial research but also of interest for labs in the biotech industry Shirley Pease heads the "Transgenic and Knockout Core Facility" at the California Institute of Technology, Pasadena, CA; Carlos Lois is at the MIT, Cambridge, MA With superb color figures demonstrating the various steps in transgenesis Includes supplementary material: sn.pub/extras

Klappentext

Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.

Inhalt
Generation of Transgenic Animals Using Lentiviral Vectors.- Intracytoplasmic Sperm Injection (ICSI) in the Mouse.- Generation of Embryonic Stem (ES) Cell-Derived Embryos and Mice by Tetraploid-Embryo Complementation.- Cloning The Laboratory Mouse by Nuclear Transfer.- Large Insert Transgenesis.- Regional and Temporal Control of Genetic Manipulation in the Mouse.- High Resolution Gene Expression Analysis in Mice Using Genetically Inserted Reporter Genes.- Nuclear Transfer in the Cow.- Production of Transgenic Pigs by DNA Microinjection.- Production of Transgenic Birds Using Lentiviral Vectors.- Ancillary Techniques.