This volume describes the protocols for fabrication of liposomal drug delivery system, and consists of two parts. The first part emphasizes the basic protocols and concepts for fabrication of drug-loaded liposomes. For new investigators, laying the groundwork is the first step. This part focuses on the fabrication details in liposomes formulations, which are the so-called small tricks while usually not disclosed in mostly published research articles. However, these processing details are crucial for the preparation of liposomes. And the second part focuses on the strategies in modified and/or functionalized liposomes to adapt to pathophysiological environment, as well as their application in treating diseases. As new analytical and synthetic technologies become available, and improved understanding of pathophysiology, various functionalized drug liposomes are developing to fit the requirements of different therapeutic purposes, exhibiting a broad range of applications. Accordingly,the objectives of this part are aimed at deeply unearthing the formulation of drug-loaded liposomes, describing the detailed operation of liposomal formulation, and further demonstrating their potential applications. Therefore, this volume shows the features of experimental report or protocol for proving a guide to scientists, research students, and young investigators in pharmaceutical enterprises.

Offers deep coverage on all aspects of biomaterials science Includes a collection of golden experimental procedures to illustrate the different fundamental concepts Provides an essential source for academics and practitioners

Autorentext
Prof. Dr. Wan-Liang Lu a principal investigator in State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Systems, and School of Pharmaceutical Sciences, Peking University, Beijing, China

Prof. Dr. Xian-Rong Qi a principal investigator in Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Systems, Department of Pharmaceutics, and School of Pharmaceutical Sciences, Peking University, Beijing, China

Inhalt
;Liposomes in Drug Delivery: Status and Advances;

Preparation of Drug Liposomes by Thin-Film Hydration and Homogenization;

Preparation of Drug Liposomes by Reverse-Phase Evaporation;

Preparation of Drug Liposomes by EDTA Gradient Methods;

Preparation and Characterization of Drug Liposomes by pH-Gradient Method;

Preparation and Characterization of Drug Liposomes by Ammonium Sulfate Gradient;

Preparation and Characterization of Drug Liposomes by Nigericin Ionophore;

Lipid-Coated Cisplatin Nanoparticles for Insoluble Drug Loading;

Purification Method of Drug-Loaded Liposome;

Quality Evaluation of Drug-Loaded Liposomes;

Coupling Methods of Antibodies and Ligands for Liposomes;

Preparation of Cell Penetrating Peptides-Mediated Targeting Drug Liposomes;

Preparation and Evaluation of Folate Receptor Mediated Targeting Liposomes;

Preparation and Evaluation of Rivastigmine Liposomes for Intranasal Delivery;

Preparation and Evaluation of Integrin Receptor-Mediated Targeting Drug Liposomes;

Preparation and Evaluation of Biomineral-Binding Antibiotic Liposomes;

Preparation of Anthracyclines Liposomes for Tumor-Targeting Drug Delivery;

Preparation and Characterization of pH Sensitive Drug Liposomes;

Preparation and Characterization of DNA Liposomes Vaccine; HER2-Specific PEGylated Immunoliposomes Prepared by Lyophilization/Rehydration Method; Fabrication and Evaluation of Dual Peptides-Modified Liposomes Coencapsulating siRNA and Docetaxel;

Dual-Modified siRNA-Loaded Liposomes for Prostate Cancer Therapy;

Dequalinium-Mediated Mitochondria-Targeting Drug Liposomes for the Treatment of Drug-Resistant Lung Cancer;

Preparation of Multifunctional Paclitaxel Liposomes forTreatment of Brain Glioma;

Preparation of Functional Vincristine Liposomes for Treatment of Invasive Breast Cancer;

Application of Labeled Liposomes in Imaging and Biodistribution Observation;

Application of Drug Liposomes in the Hormone Therapy;

Application of Drug Liposomes in Gene Transfection