Lipidomics

International experts present both reviewed and unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics.

Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols , an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures. Volume 2 focuses on oxidized lipids, trafficking and profiling, software, bioinformatics, and biostatistics. Written in the highly successful Methods in Molecular Biology ™ series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.

Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.

Regarding Volume 1 and 2: Contains in-depth guides to isolation and processing of lipid samples, probes, lipid profiling, signaling, transport, and mapping Provides examples utilizing key model systems Presents oxidized lipids obtained from in vitro and in vivo biological material, redox platforms, high throughput systems, microscopy and imaging, and computer simulation Serves as a comprehensive guide to technical applications Explores bioinformatics strategy and statistical models for standardization of lipids Includes supplementary material: sn.pub/extras

Inhalt
Oxidized Lipids.- F2-Isoprostanes: Sensitive Biomarkers of Oxidative Stress In Vitro and In Vivo: A Gas Chromatography-Mass Spectrometric Approach.- Volatile Oxylipins and Related Compounds Formed Under Stress in Plants.- Quantification of Lysophosphatidylcholine Species by High-Throughput Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS).- Use of Lipidomics for Analyzing Glycerolipid and Cholesteryl Ester Oxidation by Gas Chromatography, HPLC, and On-line MS.- Lipid Raft-Redox Signaling Platforms in Plasma Membrane.- Mass Spectrometry Analysis of Polyisoprenoids Alcohols and Carotenoids via ESI(Li+)-MS/MS.- Detection of a Lipid-Lysine Adduct Family with an Amide Bond as the Linkage: Novel Markers for Lipid-Derived Protein Modifications.- Assessing the Neuroprotective Effect of Antioxidative Food Factors by Application of Lipid-Derived Dopamine Modification Adducts.- Mass-Spectrometric Characterization of Phospholipids and Their Hydroperoxide Derivatives In Vivo: Effects of Total Body Irradiation.- Trafficking and Profiling.- Comprehensive mRNA Profiling of Lipid-Related Genes in Microglia and Macrophages Using Taqman Arrays.- Imaging Lipid Membrane Domains with Lipid-Specific Probes.- Monitoring Sterol Uptake, Acetylation, and Export in Yeast.- Methods to Monitor Fatty Acid Transport Proceeding Through Vectorial Acylation.- Activity-Based Profiling of Lipases in Living Cells.- Histochemistry and Lipid Profiling Combine for Insights into Aging and Age-Related Maculopathy.- Software and Bioinformatics.- Instrument-Independent Software Tools for the Analysis of MSMS and LCMS Lipidomics Data.- Computer-Assisted Interpretation of Triacylglycerols Mass Spectra.- Visualization of Complex Processes in Lipid Systems Using Computer Simulations and MolecularGraphics.- Bioinformatics Strategies for the Analysis of Lipids.- Biostatistics.- The Effect of Lipid Adjustment on the Analysis of Environmental Contaminants and the Outcome of Human Health Risks.