The meeting on "Molecular Mechanisms of Lipid and Protein Traffic", sponsored by NATO Scientific Affairs Division as an Advanced Study Institute and by the Federation of European Biochemical Societies as a Lecture Course was held in Cargese, France, in June 1997. The program included introductory lectures, specialized up-to-date contributions, poster sessions, tutorials and workshops. Emphasis was laid on the new developments in the field of membrane dynamics, in particular on the insertion and translocation of proteins; on intracellular protein, lipid and membrane traffic and sorting and on the mutual interactions between the various events occurring during membrane biogenesis. Mitochondrial biogenesis, chloroplast assembly and the insertion of proteins into peroxisomes were highlighted. Much progress in these research areas has been made in recent years and the ASI provided an excellent opportunity to illustrate this progress in comparison with previous meetings on a similar topic. Not only graduate students and postdocs took advantage from this program but also experienced scientists were given the opportunity to obtain a complete overview of recent progress and the remodeling of ideas and concepts.

A complete overview of recent progress in the field

Klappentext

This volume focusses on new developments in the field of membrane dynamics, in particular on the insertion and translocation of proteins, on intracellular protein, lipid and membrane traffic and sorting, and on the mutual interactions between the various events occurring during membrane biogenesis. Mitochondrial biogenesis, chloroplast assembly and the insertion of proteins into peroxisomes are highlighted. Toxins acting both from the intracellular as well as from the outside on membranes are treated including the effects of these compounds on membrane pores, channels and conductance. Lipid structure and function are discussed in relation to protein insertion into and translocation through membranes and the mechanisms underlying intracellular sorting and trafficking of specific phospholipids are elucidated.

Inhalt
Import, Folding and Degradation of Proteins by Mitochondria.- Protein Complexes Involved in Import of Mitochondrial Preproteins.- Protein Targeting into and within Chloroplasts.- Chaperone Action in Folding Newly-Translated Cytosolic Proteins in Bacteria and Eukaryotes.- Biogenesis of Peroxisomes.- Molecular Dissection of Clathrin Coat Formation and Receptor Sorting.- A Function for EGF-Induced Eps 15 Ubiquitination in Endocytosis.- The Role of ?2Macroglobulin Receptor Associated Protein as a Chaperone for Multifunctional Receptors.- Characterization of the Gene VII and Gene IX Minor Coat Proteins from Bacteriophage M13.- Lipoprotein Sorting in Escherichia coli.- In Vitro Studies of the Interactions between Signal Peptides and Signal Recognition Factors.- A PreS1-Specific Sequence Determines the Dual Topology of the Hepatitis B Virus Large Envelope Protein and Binds the Heat Shock Protein Hsc70.- Characterization of Components of the General Secretion Pathway of Aeromonas hydrophila.- Do the Type I Signal Peptidases of Bacillus subtilis Compete for Binding and Cleavage of Secretory Precursor Proteins?.- Studies on N-Glycosylation of Proteins in the Endoplasmic Reticulum.- Role of COP Coats and GTPases in Transport of Cargo through the Early Secretory Pathway.- Mapping the Golgi Retention Signal in the Cytoplasmic Tail of the Uukuniemi Virus G1 Glycoprotein.- REP-Mediated Protein Prenylation.- Development of a Positive Screen for the Identification of Suppressive Mutations in Secretion Defective Strains of Pseudomonas aeruginosa.- Mitotic Division of the Golgi Apparatus.- Vacuole Inheritance: A New Window on Interorganelle Traffic.- Sea Urchin Nuclear Envelope Assembly in Vitro.- Cell Vesicle Trafficking and Bacterial Protein Toxins.- Pore-Forming Antimicrobial Peptidesand Polypeptides: Mechanisms of Insertion into Membranes and Mode of Action.- Potassium Channels in Escherichia coli.- A Link between Fatty Acid Synthesis and Organelle Structure in Yeast.- Lipid Translocation from the Cytosolic Leaflet of the Plasma Membrane to the Cell Surface by Multidrug Transporters.- Mycobacterial Glycopeptidolipid Interactions with Membranes: an Air-Water Monolayer Study by FTIR Spectroscopy.- Consequences of Hydrophobic Matching on the Lateral Distribution of Lipids around Bacteriorhodopsin Reconstituted in DLPC/DSPC Mixtures.- Translocation of Phospholipids between the Endoplasmic Reticulum and Mitochondria in Yeast.- Lipid Peroxidation in Myocardial Cells under Ischemia and Oxidative Stress. The Effect of Allopurinol and Oxypurinol.