This volume provides researchers with a comprehensive guide for understanding light-sheet fluorescence microscopy (LSFM), and covering issues in both its routine use of the method and the latest advancements. The chapters in this book are organized into three parts: Part One is an introduction to the method, its history, and a review on tissue clearing. Part Two focuses on recent advancements in LSFM that cover increasing resolution, field of view, and image contrast. Part Three presents several guides to the use of LSFM in an imaging facility, human brain imaging, mapping of immediate early genes, and in vivo functional imaging on Zebrafish. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and thorough, Light-Sheet Microscopy is a valuable resource for all researchers who are interested in learning more about the basic and advanced uses of LSFM.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

The Development of Ultramicroscopy.- Tissue Clearing.- Advanced Tissue Clearing and Axially Swept Light-Sheet Microscopy for High-Resolution Imaging of Neural Systems.- Light-Sheet Fluorescence Microscopy of Expanded Neuronal Tissue.- Expansion Lattice Light-Sheet Microscopy Reveals Subcellular Organization and Molecular Profile of the Brain with Nanoscopic Resolution.- A Guide to Leveraging Electronics Metrology Technologies for New Light-Sheet Microscopy Systems.- RAPID Autofocusing in Light-Sheet Microscopy: An Implementation Guide.- Light-Sheet in a Multi-User Facility.- Cell-Resolution Human Brain Reconstruction with Light-Sheet Fluorescence Microscopy.- BRANT Pipeline to Map Neuronal Activations Across the Entire Brain: An Implementation Guide.- High Spatio-Temporal Resolution Functional Imaging of the Entire Larval Zebrafish Brain with Two-Photon Light-Sheet Fluorescence Microscopy.