Interdisciplinary in approach, this definitive text on viral/nuclear interactions illustrates how viruses (positive and negative DNA and RNA) interact with the nucleus and its components to further viral replication, and the resulting consequences for the host cell. The first text to present a global overview of this topic, Viruses and the Nucleus provides an accessible introduction to this increasingly popular research area.

Informationen zum Autor Dr. Julian Alexander Hiscox , School of Biochemistry and Molecular Biology, University of Leeds, UK. Klappentext Viruses have limited genome-coding capacities and must therefore rely on their host cells to facilitate every step of the infection cycle from the replication of their genomes, transcription and translation of mRNAs to virus assembly. Aimed at virologists and cell biologistsViruses and the Nucleus provides a comprehensive and cohesive overview of this fascinating and fast moving field. It compares and contrasts the ways in which DNA viruses, retroviruses and RNA viruses interact with the host cell nucleus to bring about replication and how they subvert the host cell function to proliferate and survive.Written by a team of leading experts in the field, this multi-authored text begins with an introduction to the key nuclear process that effect virus biology including cell cycle, transcription, splicing and protein trafficking. It then goes on to explore the advances that have been made in understanding the ways in which specific viruses interact with nuclear sub-structures such as the nucleolus and ND10s, and the implications this interrelationship has for the cell cycle as a whole.Key Features* Comprehensive cross disciplinary coverage of the interrelationship between cell biology and virology.* Written by leading experts, this authorative book provides an up to date overview of this highly active field.* Covers the latest research areas including virus interactions with sub-nuclear structures, virus protein trafficking into and out of the nucleus and subversion of host-cell function through specific nuclear interactions.Viruses and the Nucleus will be an invaluable resource for students of virology, microbiology and cell biology as well as those who work within the industry. Zusammenfassung Interdisciplinary in approach, this definitive text on viral/nuclear interactions illustrates how viruses (positive and negative DNA and RNA) interact with the nucleus and its components to further viral replication, and the resulting consequences for the host cell. Inhaltsverzeichnis Contributors ix 1 The Nucleus - An Overview 1 Dean A. Jackson 1.1 Introduction 1 1.2 The gene expression pathway in mammalian cells 2 1.3 Chromosome structure and DNA loops 9 1.4 Duplicating the genetic code 9 1.5 Nuclear compartmentalization 10 1.6 Nuclear architecture - implications for viral infection 18 1.7 Conclusion 19 References 20 2 The Eukaryotic Cell Cycle 25 Jane V. Harper and Gavin Brooks 2.1 Introduction 25 2.2 The G0/G1 transition 31 2.3 The G1-S transition 32 2.4 S phase 39 2.5 The G2-M transition 45 2.6 Mitosis (M-phase) 48 2.7 Cytokinesis 52 2.8 Endoreduplication 53 2.9 Summary and conclusions 53 References 54 3 DNA Viruses and the Nucleus 69 G. Eric Blair and Nicola James 3.1 Introduction 69 3.2 The structure of the nucleus 70 3.3 DNA viruses and the nucleus 75 3.4 Concluding comments and future directions 81 References 81 4 Retroviruses and the Nucleus 89 Carlos de Noronha and Warner C. Greene 4.1 Retroviral structure 91 4.2 The retroviral life cycle 91 4.3 Entering the nucleus 93 4.4 Inside the nucleus 101 4.5 Leaving the nucleus and the cell 113 4.6 Future perspectives 114 References 114 5 Negative-sense RNA Viruses and the Nucleus 129 Debra Elton and Paul Digard 5.1 Introduction 129 5.2 The necessity of a nucleus 132 5.3 Nuclear import machinery 133 5.4 The cellular transcription machinery 135 5.5 Nuclear architecture 140 5.6 Nuclear export 143 5.7 Evasion of ...

Autorentext

Dr. Julian Alexander Hiscox, School of Biochemistry and Molecular Biology, University of Leeds, UK.

Klappentext

Viruses have limited genome-coding capacities and must therefore rely on their host cells to facilitate every step of the infection cycle from the replication of their genomes, transcription and translation of mRNAs to virus assembly. Aimed at virologists and cell biologistsViruses and the Nucleus provides a comprehensive and cohesive overview of this fascinating and fast moving field. It compares and contrasts the ways in which DNA viruses, retroviruses and RNA viruses interact with the host cell nucleus to bring about replication and how they subvert the host cell function to proliferate and survive. Written by a team of leading experts in the field, this multi-authored text begins with an introduction to the key nuclear process that effect virus biology including cell cycle, transcription, splicing and protein trafficking. It then goes on to explore the advances that have been made in understanding the ways in which specific viruses interact with nuclear sub-structures such as the nucleolus and ND10s, and the implications this interrelationship has for the cell cycle as a whole. Key Features * Comprehensive cross disciplinary coverage of the interrelationship between cell biology and virology. * Written by leading experts, this authorative book provides an up to date overview of this highly active field. * Covers the latest research areas including virus interactions with sub-nuclear structures, virus protein trafficking into and out of the nucleus and subversion of host-cell function through specific nuclear interactions. Viruses and the Nucleus will be an invaluable resource for students of virology, microbiology and cell biology as well as those who work within the industry.

Inhalt

Contributors ix

1 The Nucleus - An Overview 1
Dean A. Jackson

1.1 Introduction 1

1.2 The gene expression pathway in mammalian cells 2

1.3 Chromosome structure and DNA loops 9

1.4 Duplicating the genetic code 9

1.5 Nuclear compartmentalization 10

1.6 Nuclear architecture - implications for viral infection 18

1.7 Conclusion 19

References 20

2 The Eukaryotic Cell Cycle 25
Jane V. Harper and Gavin Brooks

2.1 Introduction 25

2.2 The G0/G1 transition 31

2.3 The G1-S transition 32

2.4 S phase 39

2.5 The G2-M transition 45

2.6 Mitosis (M-phase) 48

2.7 Cytokinesis 52

2.8 Endoreduplication 53

2.9 Summary and conclusions 53

References 54

3 DNA Viruses and the Nucleus 69
G. Eric Blair and Nicola James

3.1 Introduction 69

3.2 The structure of the nucleus 70

3.3 DNA viruses and the nucleus 75

3.4 Concluding comments and future directions 81

References 81

4 Retroviruses and the Nucleus 89
Carlos de Noronha and Warner C. Greene

4.1 Retroviral structure 91

4.2 The retroviral life cycle 91

4.3 Entering the nucleus 93

4.4 Inside the nucleus 101

4.5 Leaving the nucleus and the cell 113

4.6 Future perspectives 114

References 114

5 Negative-sense RNA Viruses and the Nucleus 129
Debra Elton and Paul Digard

5.1 Introduction 129

5.2 The necessity of a nucleus 132

5.3 Nuclear import machinery 133

5.4 The cellular transcription machinery 135

5.5 Nuclear architecture 140

5.6 Nuclear export 143

5.7 Evasion of innate antiviral responses 147

References 148

6 Positive-strand RNA Viruses and the Nucleus 161
Kurt E. Gustin and Peter Sarnow

6.1 Introduction 161

6.2 Replication of positive-strand RNA viruses in enucleated cells 162

6.3 Localization of viral proteins to the nucleus 163

6.4 Interaction of nuclear factors with viral proteins and nucleic acids 165

6.5 Cytoplasmic accumulation of nuclear proteins during infection 168

6.6 Disruption of nucleo-cytoplasmic trafficking by positive-strand RNA viruses 169

6.7 Advantages provided to the virus by inhibiting nuclear import …