In Lipoprotein Protocols, Jose Ordovas compiles a cutting-edge collection of molecular protein techniques for studying advanced aspects of lipoprotein structure and metabolism. These step-by-step methods allow both the beginning and the advanced researcher to carry out not only fundamental, but also the most advanced genetic screening techniques, including RT-PCR and the preparation of relevant models of transgenic and knockout mice. In addition, novel techniques are presented to characterize plasma lipoproteins, their subfractions, and their modifications, and to determine the activity of relevant enzymes and carrier proteins. Each method is described in easily reproducible detail by hands-on experts who have perfected it.

Lipoprotein Protocols offers many powerful tools for unraveling the complexity of lipoprotein metabolism and determining the individual risk of coronary heart disease. For
all those basic and clinical investigators seeking to advance the frontier of lipoprotein research and develop novel genetic therapies for the treatment of cardiovascular disease, this eminently useful book constitutes the new standard reference and guide.

Klappentext

Jose Ordovas compiles a cutting-edge collection of molecular protein techniques for studying advanced aspects of lipoprotein structure and metabolism. These step-by-step methods allow both the beginning and the advanced researcher to carry out not only fundamental, but also the most advanced genetic screening techniques, including RT-PCR and the preparation of relevant models of transgenic and knockout mice. In addition, novel techniques are presented to characterize plasma lipoproteins, their subfractions, and their modifications, and to determine the activity of relevant enzymes and carrier proteins. Each method is described in easily reproducible detail by a hands-on expert who has perfected it. Lipoprotein Protocols offers many powerful tools for unraveling the complexity of lipoprotein metabolism and determining the individual risk of coronary heart disease.

Inhalt
DNA- and RNA-Based Methodolog.- PCR Methodology Applied to Genetic Studies of Lipoprotein Metabolism and Atherosclerosis.- Separation of Small-Size DNA Fragments Using Agarose Gel Electrophoresis.- Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method.- Generation of Transgenic Mice.- Preparation of Knockout Mice.- Protein-Based Methodology.- Fast Ultracentrifugation Methods for the Separation of Plasma Lipoproteins.- Preparative Isolation of Plasma Lipoproteins Using Fast Protein Liquid Chromatography (FPLC).- Separation of Apolipoproteins by Polyacrylamide Gel Electrophoresis.- Isolation and Purification of Serum Lipoprotein (a) Using Lectin Affinity.- Electrophoretic Separation of LDL and HDL Subclasses.- Measurement of Lipoprotein Particles.- Methodological Approaches for Assessing Lipid and Protein Oxidation and Modification in Plasma and Isolated Lipoproteins.- Separation and Quantitation of Phospholipid Classes by HPLC.- Assays of Lecithin Cholesterol Acyltransferase (LCAT).- Determination of the Mass Concentration and the Activity of the Plasma Cholesteryl Ester Transfer Protein (CETP).- In Vitro Measurement of Lipoprotein and Hepatic Lipases.- Cellular Assays for Lipoprotein Receptors.- Measurements of ProteoglycanLipoprotein Interaction by Gel Mobility Shift Assay.