Provides comprehensive and up-to-date knowledge on the fluorescence technology in biomedical applications.

Autorentext

Jiangli Fan is currently Professor at the State Key Laboratory of Fine Chemicals, Dalian University of Technology (China), the winner of the national outstanding youth fund and the third batch of national "ten thousand plan" scientific and technological innovation leading talents. Her research interests focus on small-molecule fluorescent probes for bioimaging and sensing, photosensitizers based photodynamic therapy, and targeted nanodrug delivery. She is now on the editorial board of Dyes and Pigments and Scientific Reports.
Xiaojun Peng is the Director of the State Key Laboratory of Fine Chemicals at Dalian University of Technology, China. His research interests cover dyes for fluorescent bioimaging/labeling and digital printing/recording. Currently, he is the editor-in-chief of Smart Molecules. He was elected as a member of Chinese Academy of Sciences in 2017.

Inhalt
CHAPTER 1 INTRODUCTION
1.1 Overview of the Development of Fluorescence and Phosphorescence
1.2 Mechanisms of Fluorescence and Phosphorescence
References

CHAPTER 2 FLUORESCENT DYES AND THEIR PROSPECTS
2. 1 Common Fluorescent Dyes
2.2 Molecular Design Rules for Near Infrared Fluorescent Dyes
2.3 Future Technology Outlook for Fluorescent Dyes
References

CHAPTER 3 ORGANELLE STAINING FLUORESCENT DYES
3.1 Overview
3.2 Cell Membrane Staining Fluorescent Dyes
3.3 Mitochondrial Staining Fluorescent Dyes
3.4 Lysosomal Staining Fluorescent Dyes
3.5 Golgi Apparatus Staining Fluorescent Dyes
3.6 Endoplasmic Reticulum Staining Fluorescent Dyes
3.7 Nuclear Staining Fluorescent Dyes
References

CHAPTER 4 CELLULAR ENVIRONMENT-SENSITIVE FLUORESCENT DYE
4.1 Polarity-sensitive Fluorescent Dyes
4.2 Temperature Sensitive Fluorescent Dyes
4.3 Viscosity Sensitive Fluorescent Dyes
4.4 pH-sensitive Fluorescent Dyes
4.5 Prospects of Environment-sensitive Fluorescent Dyes in Biomedical Applications
References

CHAPTER 5 FLUORESCENT PROBES FOR INTRACELLULAR ION IMAGING
5.1 Fluorescent Probes for Alkali and Alkaline Earth Metal Ions
5.2 Fluorescent Probe for Normal Transition Metal Ions in Organisms
5.3 Fluorescent Probes for Anions
5.4 Fluorescent Probes for Harmful Heavy Metal Ions
References

CHAPTER 6 FLUORESCENT RECOGNITION DYES FOR THE DETECTION OF BIOACTIVE SMALL MOLECULES
6.1 Introduction
6.2 Fluorescent Recognition Dyes for Reactive Oxygen Species
6.3 Fluorescent Probes for Biological Thiol Compounds Recognition
6.4 Fluorescent Probes for Gasotransmitter Recognition
References

CHAPTER 7 FLUORESCENT RECOGNITION DYE FOR BIOLOGICAL ENZYME
7.1 Introduction
7.2 Fluorescent Probe for Oxidoreductase
7.3 Fluorescent Recognition Dye for Transferase
7.4 Fluorescent Recognition Dye for Hydrolases
References

CHAPTER 8 FLUORESCENT RECOGNITION DYES FOR NUCLEIC ACIDS
8.1 Basic Characteristics of Nucleic Acids
8.2 Basic Principles and Categories of Dyeing
8.3 DNA Fluorescent Dyes
8.4 RNA Fluorescent Dyes
8.5 G tetrad Fluorescent Dyes
8.6 Multifunctional Fluorescent Probes based on Nucleic Acid Staining
References

CHAPTER 9 PROTEIN FLUORESCENT RECOGNITION
9.1 Specific Labeling Techniques
9.2 Small-molecule Ligand
9.3 Peptide Tag Labeling
9.4 Self-labeling Protein Tag
9.5 Aptamer Labeling
9.6 Summary and Outlook
References

CHAPTER 10 THERMALLY ACTIVATED DELAYED FLUORESCENCE DYES
10.1 Introduction
10.2 Structural and Spectral Properties of TADF Dyes
10.3 TADF Dyes for Fluorescence Imaging in Biology
10.4 TADF Dyes for Theranostic Anticancer
10.5 Prospects of Biomedical Applications for TADF Dyes
References

CHAPTER 11 FLUORESCENT DYES FOR SUPER-RESOLUTION IMAGING
11.1 Overview
11.2 Super-Resolution Imaging Technology
11.3 Fluorescent Dyes for Super-Resolution Imaging
11.4 Conclusion and Outlook
References

CHAPTER 12 FLUORESCENT MOLECULAR PRODRUGS
12.1 Fluorescent Molecular Prodrugs Activated by Reductive Biothiols
12.2 Fluorescent Molecular Prodrugs Activated by Hydrogen Peroxide
12.3 Fluorescent Molecular Prodrugs Activated by Enzymes
12.4 Fluorescent Molecular Prodrugs Activated by Acidic pH
12.5 Fluorescent Molecular Prodrugs Activated by External Light
References

CHAPTER 13 PHOTOSENSITIZING DYES FOR LIGHT-TRIGGERED THERAPY
13.1 Overview
13.2 Photosensitizing Dyes for Photodynamic Therapy
13.3 Photosensitizing Dyes for Photothermal Therapy
References