Pharmacological biotechnology is applied to and used to study drug development, working mechanisms, diagnosis, and therapies. This textbook covers the whole range of experiments related to pharmacology. It also contains basic laboratory safety guidelines along with the basic calculations and formulas used in a laboratory. Each chapter starts with an introduction/theory into the basic approach followed by detailed methods sections with easy-to-follow protocols and comprehensive troubleshooting, calculations and possible questions for examination. The target group is researchers who are studying pharmacological biotechnology in the laboratory.

Offers practical guidelines for experiments in pharmacological biotechnology Addresses principles with workable protocols Illustrates flow-chart and troubleshooting for each experiment

Autorentext
Dr. Jayanta Kumar Patra, M.Sc. Ph.D., is currently an Assistant Professor at Dongguk University, Republic of Korea. He has 14 years of research and teaching experience in the field of food, pharmacological and nanobiotechnology, and he has published more than 140 papers in various national and international, peer-reviewed journals, as well as 30 book chapters and 11 books. Dr. Gitishree Das, M.Sc. Ph.D., is currently an Assistant Professor at Dongguk University, Republic of Korea. She has 10 years of research experience in the field of rice molecular biology, plant breeding, endophytic bacteria and green nanotechnology, and 3 years of teaching experience. Her current research focuses on the biosynthesis of nanoparticles using food waste and plant materials and their applications in the biomedical and agricultural fields. She has published roughly 70 research articles in respected international and national reputed journals, 16 book chapters and 6 books. Dr. Swagat Kumar Das, B.Pharm., M.Tech., Ph.D., is currently a lecturer at the Department of Biotechnology at the College of Engineering and Technology, an autonomous and constituent College of Biju Patnaik University of Technology, Rourkela, India. He teaches, medical and pharmaceutical biotechnology, upstream and downstream process engineering, bioprocess engineering, and intellectual property rights at B.Tech and M.Tech level. He has more than 8 years of teaching and research experience, and has published more than 25 research and review papers along with a number of book chapters and 2 books. Prof. Hrudayanath Thatoi, M.Sc., M.Phil, Ph.D., is currently a Professor and Head of the Department of Biotechnology, North Orissa University, Odisha, India. His has 30 years of teaching and research experience, and his interests include medicinal plants, bioremediation, biodiversity, ethnopharmacology and mangrove biology. Prof. Thatoi has publisher more than 200 research papers in various respected national and International journals, as well as 30 book chapters and 10 books.

Inhalt
Part1.Introduction.- 1.1 Rules of laboratory safety in a pharmacology laboratory.- 1.2 Commom instruments and their uses used in a pharmacology laboratory.- Part2.General aspects of Pharmacology laboratory.- 2.1 Safety and Risk assessment.- 2.2 Use and Handling of laboratory animals.- 2.3 Experimental design.- 2.4 Essential statistics.- 2.5 Cumulative Dose Response Curve.- 2.6 Toxicology.- 2.7 Basic instruments and techniques in Pharmacology Laboratory .- Part3.Isolated tissues and organs.- 3.1 Basic instruments used for isolated tissue experiments.- 3.2 Organ baths.- 3.3 Smooth muscle preparations.- 3.4 Skeletal muscle preparations.- 3.5 Cardiac muscle preparations.- Part4.Isolated tissues for screening of drugs.- 4.1 Evaluation of antidiabetic agents.- 4.2 Evaluation of antidepressants.- 4.3 Evaluation of antihypertensive agents.- 4.4 Evaluation of antiulcer agents.- 4.5 Evaluation of hepatotoxicity.- 4.6 Evaluation of Antioxidant agents.- 4.7 Evaluation of local anaesthetics.-Part5.Genotoxicity and Toxicological studies.- 5.1 The Mouse Lymphoma Assay.- 5.2 The Comet Assay.- 5.3 In vitro Genotoxicity assay.- 5.4 In vitro Teratogenicity Testing.- 5.5 Histopathological studies of animal tissues.- 5.6 Drug poisoning.- Part6.Experimental Animal studies.- 6.1 Pyrogen testing.- 6.2 Collecting blood from mice.- 6.3 Studies on different parameters of blood.- 6.4 Experiment on central nervous system.- 6.5 Evaluation on cardiovascular system.- 6.6 Experiments on GI tract.- Part7.Clinical trials.- 7.1 Clinical Pharmacology and its genesis.- 7.2 National and International agencies and their role in Clinical pharmacology.- 7.3 Stages in drug development and clinical trials.- 7.4 Ethics in Clinical research.- 7.5 Safety assessment in Clinical trials.- Part8.IPR and ethics in animal studies.- 8.1 Intellectual property rights and its different categories.- 8.2 Importance of IPR in drug development.- 8.3 Patenting cells, cell lines and animals.- 8.4 Ethics in laboratory animal studies .- 8.5 Risk assessment and management.- 8.6 Good laboratory practices.