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Integrin and Cell Adhesion Molecules

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  • 529 Seiten
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This Methods in Molecular Biology(TM) volume covers basic protocols for the study of integrin and related cell adhesion molecule functionality, and showcases the most promising methods and technologies for the development of novel therapeutics and diagnostics.

Integrins play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, immune responses, wound healing, and regulation of cell growth and differentiation, but also in numerous pathological phenomena such as autoimmunity, thrombosis, and cancer metastasis/progression. Therefore, investigations on integrins often demand multi-disciplinary approaches, making researchers long for a handy collection of comprehensive and practical protocols that detail experimental methods for studying integrin and related cell adhesion molecule functionality. Integrin and Cell Adhesion Molecules: Methods and Protocols aims to provide readers not only with basic protocols in studying integrin functions, but also with summaries on those state-of-the-art technologies that have been utilized for understanding integrin functionality at the cellular, molecular, structural, and organismal levels. Divided into six convenient sections, this detailed volume covers basic protocols for the study of integrin and related cell adhesion molecule functionality in vitro, illustrates structural biology approaches for studying integrins and related cell adhesion molecules, focuses on emerging imaging technologies for investigating cell migration, presents strategies to elucidate signaling through cell adhesion molecules, includes experimental techniques to investigate integrin functions at organismal levels in a physiological context, and showcases the most promising methods and technologies for the development of novel therapeutics and diagnostics. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Both experts and non-experts in the scientific community who wish to study cell adhesion molecules and diagnostics will find Integrin and Cell Adhesion Molecules: Methods and Protocols authoritative, easily accessible, and vastly informative.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts


Part I: Basic Protocols for the Study of Integrin and Related Cell Adhesion Molecule Functionality In Vitro

1. Overview: Assays for Studying Integrin-Dependent Cell Adhesion

Alexandre Chigaev and Larry A. Sklar

2. Cell Adhesion Assays

Gabriele Weitz-Schmidt and Stéphanie Chreng

3. Real Time Analysis of Integrin-dependent Transendothelial Migration and Integrin-independent Interstitial Motility of Leukocytes

Ziv Shulman and Ronen Alon

4. Lentiviral Gene Transfer Method to Study Integrin Function in T Lymphocytes

Daliya Banerjee and Motomu Shimaoka

5. Surface Plasmon Resonance Biosensing in Studies of the Binding between b2 Integrin I Domains and their Ligands

Thomas Vorup-Jensen

6. Cell-Free Ligand-Binding Assays for Integrin LFA-1

Koichi Yuki

Part II: Structural Biology Approaches for Studying Integrins and Related Cell Adhesion Molecules

7. Overview: Structural Biology of Integrins

Guanyuan Fu, Wei Wang, and Bing-Hao Luo

8. Protein Expression and Purification of Integrin I Domains and IgSF Ligands for Crystallography

Hongmin Zhang and Jia-huai Wang

9. Electron Microscopic Imaging of Integrin

Kenji Iwasaki

10. An NMR Method to Study Protein-Protein Interactions

Noritaka Nishida and Ichio Shimada

11. Single Molecule Methods to Study Cell Adhesion Molecules

Joonil Seog

Part III: Imaging for Investigating Cell Adhesion and Migration

12. Overview: Imaging in the Study of Integrins

Christopher V. Carman

13. Live Imaging of LFA-1-dependent T Cell Motility and Stop Signals

Andrew J. Wiemer, Sarah Wernimont, and Anna Huttenlocher

14. Monitoring Integrin Activation by Fluorescence Resonance Energy Transfer

Craig T. Lefort, Young-min Hyun, and Minsoo Kim

15. High-resolution Fluorescence Microscopy to Study Transendothelial Migration

Christopher V. Carman

16. Multiphoton Intravital Microscopy to Study Lymphocyte Motility in Lymph Nodes

Thomas T. Murooka and Thorsten R. Mempel

Part IV: Signaling through Cell Adhesion Molecules

17. Overview of Integrin Signaling in the Immune System

Tatsuo Kinashi

18. Rap1 and Integrin Inside-out Signaling

Koko Katagiri and Tatsuo Kinashi

19. Isolation of Focal Adhesion Proteins for Biochemical and Proteomic Analysis

Jean-Cheng Kuo, Xuemei Han, John R. Yates III, and Clare M. Waterman

20. Talin and Signaling Through Integrins

Mohamed Bouaouina, David S. Harburger, and David A. Calderwood

21. Proteomics Method for Identification of Pseudopodium Phosphotyrosine Proteins

Yingchun Wang and Richard L. Klemke

Part V: Cell Adhesion and Migration at Organismal Levels in a Physiological Context

22. Overview: Studying Integrins In Vivo

Clifford A. Lowell and Tanya N. Mayadas

23. A Method for the Generation of Conditional Gene-Targeted Mice

Masahiro Yamamoto and Kiyoshi Takeda

24. T Cell Homing to the Gut Mucosa: General Concepts and Methodological Considerations

Jaime De Calisto, Eduardo J. Villablanca, Sen Wang, Maria R. Bono, Mario Rosemblatt, and J. Rodrigo Mora

25. In Vivo Quantitative Proteomics: the SILAC-Mouse

Sara Zanivan, Marcus Krueger, and Matthias Mann

26. Analysis of Chemotaxis in Dictyostelium

Huaqing Cai, Chuan-Hsiang Huang, Peter N. Devreotes, and Miho Iijima

Part VI: Methods and Technologies towards Novel Therapeutics and Diagnostics Targeting Integrins

27. Integrins in Tumor Angiogenesis & Lymphangiogenesis

Philippe Foubert and Judith A. Varner

28. PET-Radioimmunodetection of Integrins: Imaging Acute Colitis Using a 64Cu-Labeled Anti-b7 Integrin Antibody

Jason L.J. Dearling and Alan B. Packard

29. Integrin-targeted Nanoparticles for siRNA Delivery

Noa Ben-Arie, Ranit Kedmi, and Dan Peer

30. Humanized Mice for Studying Human Leukocyte Integrins In Vivo

Sang-Soo Kim, Priti Kumar, and Premlata Shankar


Titel: Integrin and Cell Adhesion Molecules
Untertitel: Methods and Protocols
EAN: 9781617791659
ISBN: 1617791652
Format: Fester Einband
Herausgeber: Springer-Verlag GmbH
Veröffentlichung: 01.10.2011
Anzahl Seiten: 529
Gewicht: 1215g
Größe: H260mm x B183mm x T32mm
Jahr: 2011
Untertitel: Englisch
Auflage: 2012

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