Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Detection of RNA Modifications by HPLC Analysis and Competitive ELISA.- Enzymatic Synthesis and Purification of a Defined RIG-I Ligand.- Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis.- Isolation of RIG-I-associated RNAs from Virus Infected Cells.- Structure Modeling of Toll-like Receptors.- Nucleic Acid Recognition in Dendritic Cell.- Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems.- Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro.- In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes.- Mapping of Optimal CD8 T Cell Epitopes.- A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium and Viral Infection.- MicroRNA Methodology: Advances in miRNA-technologies.- Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR).- Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus.- Induction and Analysis of Nephrotoxic Serum Nephritis in Mice.- Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice.- Bifunctional siRNAs for Tumor Therapy.