This volume aims to be a collection of essential protocols in iPS cell field. Induced Pluripotent Stem (iPS) Cells: Methods and Protocols *guides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written for the Methods in Molecular Biology* series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols provides protocols that can be used by both experts and novices interested in stem cells.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
The piggyBac Transposon as a Platform Technology for Somatic Cell Reprogramming Studies in Mouse .- Vallier Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus.- Virus-based Reprograming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton's Jelly into Induced Pluripotent Stem Cells.- Very Rapid and Efficient Generation of Induced Pluripotent Stem Cells from Mouse pre-B Cells.- Generation of iPS cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors.- Enhancing Induced Pluripotent Stem Cell Generation by MicroRNA.- Generation of Partially Reprogrammed Cells and Fully Reprogrammed iPS Cells By Plasmid Transfection.- Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts to Induced Pluripotent Stem Cells (iPSCs).- Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.- Generation and Characterization of Rat iPSCs.- Generation of Induced Pluripotent Stem Cells in Rabbits.- Monkey Induced Pluripotent Stem Cells Generated by Using Allogeneic Genes.- Induced Pluripotent Stem Cells from Nonhuman Primates.- Computational Biology Methods for Characterization of Pluripotent Cells.- cGMP-compliant Expansion of Human iPSC Cultures as Adherent Monolayers.- Analysis of the Mitochondrial DNA and its Replicative Capacity in Induced Pluripotent Stem Cells.- Selection of Phage Display Peptides Targeting Human Pluripotent Stem Cell Derived Progenitor Cell Lines.- piggyBac Transposon Mediated Reprogramming and Flow Cytometry Analysis of CD44 and ICAM1 Cell-Surface Marker Changes.- Generation and in vitro Expansion of Hepatic Progenitor Cells from Human iPS Cells.- Induced Pluripotent Stem (iPS) Cell Culture Methods and Induction of Differentiation into Endothelial Cells.- Derivation of Neural Stem Cells from Mouse Induced Pluripotent Stem Cells. Production of Retinal Cells from Confluent Human iPS Cells.- Differentiation of iPSC to Mesenchymal Stem-Like Cells and their Characterization.- Hepatic Differentiation from Human iPS Cells using M15 Cells.- Determination of Functional Activity of Human iPSC-derived Hepatocytes by Measurement of CYP Metabolism.- Mesoderm Differentiation from hiPS Cells.- Enhancing Human Cardiomyocyte Differentiation from Induced Pluripotent Stem Cells with Trichostatin-A.- Derivation of Skeletal Myogenic Precursors from Human Pluripotent Stem Cells Using Conditional Expression of PAX7.- Chondrogenic and Osteogenic Induction from iPS Cells.- Generation of iPS Cells from Granulosa Cells.- The Characteristics of Murine iPS Cells and siRNA Transfection under Hypoxia.- Hepatic Differentiation from Murine and Human iPS cells using Nanofiber Scaffolds.