In vivo NMR Imaging

Nuclear magnetic resonance imaging represents a technique that is indispensable in every day biomedical diagnostics. Thanks to the numerous ways to manipulate and detect an NMR signal, it is possible to obtain a variety of information with excellent spatial and temporal resolution. Today's MRI techniques go far beyond the illustration of pure anatomical structures and include the revealing of processes down to the molecular level. The number of small animal imaging centers relying on MRI as a key method for preclinical research to understand diseases and to test for novel treatments is growing rapidly. In Vivo NMR Imaging: Methods and Protocols is written as an experimental laboratory text to provide a descriptive approach of the various applications of magnetic resonance imaging and its underlying principles. Starting from a compact introduction of basic NMR physics and image encoding techniques suitable for a broad audience in the life sciences, the concept focuses on addressing the many ways of generating contrast in MR images. The authors cover an interdisciplinary range of problems to be addressed by this non-invasive modality, including study protocols for addressing morphological, physiological, functional, and biochemical aspects of various tissues in living organisms. Information about practical aspects of designing experimental studies that follow the special conditions for micro imaging setups are also provided.

Written in the successful Methods in Molecular Biology ™ series format, In Vivo NMR Imaging: Methods and Protocols aims to be an experimental compendium of modern in vivo MR imaging with special focus on recent developments in molecular imaging and new protocols for imaging metabolism and molecular markers.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Physical Basics of NMR.- Spatial Encoding - Basic Imaging Sequences.- Basic Contrast Mechanisms.- Scanner Components.- Small Animal Preparation and Handling in MRI.- Cerebral Perfusion MRI in Mice.- High Field Diffusion Tensor Imaging in Small Animals and Excised Tissue.- The BOLD Effect.- Screening of CEST MR Contrast Agents.- Hyperpolarized Noble Gases as Contrast Agents.- Hyperpolarized Molecules in Solution.- MR Oximetry.- MRI using Intermolecular Multiple-Quantum Coherences.- Experimental Stroke Research: The Contributions of In vivo MRI.- Volumetry and Other Quantitative Measurements to Assess the Rodent Brain.- Models of Neurodegenerative Disease - Alzheimer's Anatomical and Amyloid Plaque Imaging.- MRI in Animal Models of Psychiatric Disorders.- Spectroscopic Imaging of the Mouse Brain.- Spinal Cord MR of Rodent Models.- Assessment of Cardiac Function.- Plaque Imaging in Murine Models of Cardiovascular Disease.- Interventional MRI in the Cardiovascular System.- MR for the Investigation of Murine Vasculature.- MRI of the Lung: Non-invasive Protocols and Applications to Small Animal Models of Lung Disease.- Characterization of Tumor Vasculature in Mouse Brain by USPIO Contrast-enhanced MRI.- Cancer Models - A Multiparametric Application of MRI in Rodent Hepatic Tumor Model.- BOLD MRI Applied to a Murine Model of Peripheral Artery Disease.- Manganese-enhanced Magnetic Resonance Imaging.- Spin Echo BOLD fMRI on Songbirds.- MRI to Study Embryonic Development.- Mouse Phenotyping with MRI.- Analysis of Freshly Fixed and Museum Invertebrate Specimens using High-resolution, High-throughput MRI.- Applications of Hyperpolarized Agents in Solutions.- Target-specific Paramagnetic and Superparamagnetic Micelles for Molecular MR Imaging.- Tracking Transplanted Cells by MRI - Methods and Protocols.- MRI of CEST Based Reporter Gene.