This book provides a unique selection of essays by leading experts who address all aspects of modern imaging, from its application and up-scaling to its development. Coverage ranges from the basic through to complex overview of method and protocols and also offers practical and detailed "how-to" content on important, but rarely addressed topics. The book features all-color-plate chapters and a unique continuously updated website forum where contributors and readers can share software utilities, including a limited license version of the imaging platform management software "PPMS" version 1.0 release, plus relevant new publications and links to other important resources.

Presents a unique selection of methods and applications orientated chapters on topics of interest for anyone involved in live cell microscopy Includes information that will impact both novice and expert alike Chapter content ranges from the basic through to complex overview of method and protocols Also includes practical and detailed "how-to" content on important, but rarely addressed topics Includes supplementary material: sn.pub/extras

Klappentext

'Imaging cellular and molecular biological function' provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. Indeed the philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions.

Inhalt
Considerations for Routine Imaging.- Entering the Portal: Understanding the Digital Image Recorded Through a Microscope.- Quantitative Biological Image Analysis.- The Open Microscopy Environment: A Collaborative Data Modeling and Software Development Project for Biological Image Informatics.- Design and Function of a Light-Microscopy Facility.- Advanced Methods and Concepts.- Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls.- Quantitative FRET Microscopy of Live Cells.- Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologies To Study Molecular Dynamics in Living Cells.- Single Fluorescent Molecule Tracking in Live Cells.- From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteins.- Cutting-Edge Applications and Utilities.- Towards Imaging the Dynamics of Protein Signalling.- New Technologies for Imaging and Analysis of Individual Microbial Cells.- Imaging Parasites in Vivo.- Computer-Assisted Systems for Dynamic 3D Reconstruction and Motion Analysis of Living Cells.- High-Throughput/High-Content Automated Image Acquisition and Analysis.- Cognition Network Technology A Novel Multimodal Image Analysis Technique for Automatic Identification and Quantification of Biological Image Contents.- High-Content Phenotypic Cell-Based Assays.