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Human Pluripotent Stem Cells

  • Kartonierter Einband
  • 488 Seiten
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This authoritative book is as an ideal guide to scientists conducting their own pluripotent cell research programs. The book aims ... Weiterlesen
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Beschreibung

This authoritative book is as an ideal guide to scientists conducting their own pluripotent cell research programs. The book aims to assist those making strides in this field towards furthering human knowledge and, ultimately, improving the human condition.


Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.



Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts



Zusammenfassung
Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models;

Inhalt
Section I. Laboratory Essentials1. The Stem Cell Laboratory: Design, Equipment, and Oversight Robin L. Wesselschmidt and Philip H. Schwartz 2. Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research Lesley Young, Lyn Healy, and Glyn N. Stacey Section II. Derivation 3. Embryonic Stem Cell Derivation from Human Embryos Paul Lerou 4. Derivation of Human Parthenogenetic Stem Cell Lines Nikolay Turovets, Andrey Semechkin, Leonid Kuzmichev, Jeffrey Janus, Larissa Agapova, and Elena Revazova 5. Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfer Justine D. Miller and Thorsten M. Schlaeger 6. Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz 7. Transgene-free Production of Pluripotent Stem Cells using PiggyBac Transposons Knut Woltjen, Riikka Hämäläinen, Mark Kibschull, Maria Mileikovsky, and Andras Nagy Section III. Growth, Maintenance and Expansion 8. Traditional Human Embryonic Stem Cell Culture Philip H. Schwartz, David J. Brick, Hubert E. Nethercott, and Alexander E. Stover 9. Xeno-Free Culture of Human Pluripotent Stem Cells Rosita Bergström, Susanne Ström, Frida Holm, Anis Feki, and Outi Hovatta10. Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically-Defined Medium with Enzymatic Single-Cell Passaging Alexander E. Stover and Philip H. Schwartz 11. GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications Lara J. Ausubel, Patricia M. Lopez, and Larry A. Couture 12. Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging Victoria Fox Section IV. Characterization 13. Classical Cytogenetics: Karyotyping Techniques Steven E. Bates 14. FISH Analysis of Human Pluripotent Stem Cells Suzanne E. Peterson, Jerold Chun, and Jeanne Loring 15. Immunocytochemical Analysis of Human Pluripotent Stem Cells Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz 16. Flow Cytometric Analysis of Human Pluripotent Stem Cells Mirabelle SH. Ho, Andrew Fryga, and Andrew L. Laslett 17. The Teratoma Assay: An In vivo Assessment of Pluripotency Robin L. Wesselschmidt 18. Detection of Copy Number Variation using SNP Genotyping Gregory M. Cooper and Heather C. Mefford 19. Genome-wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq Michael J. Hitchler and Judd C. Rice20. Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays Bernhard Schuldt, Qiong Lin, Franz-Josef Müller, and Jeanne F. Loring 21. Development of High Content Screening Approaches and Analysis for Human Pluripotent Stem Cells Sean P. Sherman, Jackelyn A. Alva, Kaushali Thakore-Shah, and April D. Pyle 22. Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells Javier Muñoz and Albert J. R. Heck Section V. Genetic Manipulation (Gene Modulation) 23. Lentivirus-mediated Modification of Pluripotent Stem Cells Ruchi Bajpai 24. Nucleofection of Human Embryonic Stem Cells Helen Fong, K. A. Hohenstein Elliott, Leslie F. Lock, and Peter J. Donovan 25. Non-viral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells Sujoy K. Dhara, Anirban Majumder, Mahesh C. Dodla, and Steven L. Stice 26. Gene Targeting in Human Pluripotent Stem Cells Ying Liu and Mahendra Rao 27. Episomal Transgene Expression in Pluripotent Stem Cells Michele M.P. Lufino, Anna R. Popplestone, Sally A. Cowley, Pauline A.H. Edser, William S. James, and Richard Wade-Martins Section VI: Differentiation 28. The Generation of Embryoid Bodies from Feeder-based or Feeder-free Human Pluripotent Stem Cell Cultures Alexander E. Stover and Philip H. Schwartz 29. Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells Jason Sharp, Maya Hatch, Gabriel Nistor, and Hans Keirstead 30. Directed Differentiation of Dopamine Neurons From Human Pluripotent Stem Cells Lixiang Ma, Yan Liu, and Su-Chun Zhang 31. Methods for the Derivation and Use of Cardiomyocytes from Human Pluripotent Stem Cells Wei-Zhong Zhu, Benjamin Van Biber, and Michael A. Laflamme 32. In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells Melinda K. Hexum, Xinghui Tian, and Dan S. Kaufman 33. Differentiation of Dendritic Cells from Human Embryonic Stem Cells Kathryn M. Silk, Su-Yi Tseng, Kevin P. Nishimoto, Jane S. Lebkowski, Anita Reddy, and Paul J. Fairchild

Produktinformationen

Titel: Human Pluripotent Stem Cells
Untertitel: Methods and Protocols
Editor:
EAN: 9781493958023
ISBN: 149395802X
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 488
Gewicht: 909g
Größe: H254mm x B178mm x T26mm
Jahr: 2016
Untertitel: Englisch
Auflage: Softcover reprint of the original 1st ed. 2011

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