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Hepatitis C

  • Fester Einband
  • 484 Seiten
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By providing a compilation of cutting-edge research techniques currently used in HCV labs worldwide, this book fully updates the p... Weiterlesen
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By providing a compilation of cutting-edge research techniques currently used in HCV labs worldwide, this book fully updates the previous edition. It reflects the progress which promises to unlock the remaining vital information regarding this human pathogen.

When I was ?rst approached by Dr. Hengli Tang about writing the foreword to this book, the second edition of Hepatitis C Virus, I was a little puzzled: Book forewords are penned by persons far more famous than I am. Surely there were better people to write this, with a handful of the true visionaries in the ?eld immediately coming to my mind. However, after some thought and self-re?ection, I realized that I might just be the right person for the job. I remember the publication of the ?rst edition of Hepatitis C Virus in 1998, the year I began reading about hepatitis C virus with great interest. I purchased a copy of the ?rst edition from my meager graduate student stipend and read it from cover to cover, along with a variety of hepatitis C publications, to try to get a feel if this hepatitis C virus ?eld was something I might want to work on in the future. At the time, the ?rst edition was among the most comprehensive collection of hepatitis C protocols and methods available in one volume. I was amazed at the progress that had been made in hepatitis C research, and even more amazed as I learned more about the experimental challenges facing the ?eld at that time.

Provides the most up-to-date technical information on the new cell culture infection system as written by leading experts in the field

Presents both an overview and detailed protocols on the replicon system used by virtually all the HCV labs around the world

Covers a wide range of multi-discipline approaches adopted for HCV research, including NMR-based structure determination and mathematical modelling

Broadly applicable guides valuable for studying the replication of other positive-strand RNA viruses


With the development of robust cell-culturing systems capable of Hepatitis C Virus replication and infection, the past decade has witnessed an explosion of innovative research directed at understanding HCV biology in the context of the authentic replicative cycle of the virus. By providing a compilation of cutting-edge research techniques currently in use in HCV labs worldwide, Hepatitis C: Methods and Protocols, Second Edition fully updates the previous edition to reflect this progress which promises to unlock the remaining vital information regarding this human pathogen and its interactions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Fully updated and comprehensive, Hepatitis C: Methods and Protocols, Second Edition serves as a valuable reference guide for basic and clinical researchers using HCV as a model and striving to better understand this critical disease.

PART I: DETECTION AND QUANTIFICATION OF HCV 1. Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays Hélène Le Guillou-Guillemette and Françoise Lunel-Fabiani 2. Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen Filippo Ansaldi and Giancarlo Icardi 3. Immunohistochemical Detection of HCV Proteins in Liver Tissue Oivier Galy, Isabelle Vincent, Philippe Chevallier, Nicole Lyandrat, and Isabelle Chemin PART II: HCV NONMENCLATURE, GENOTYPING, AND QUASISPECIES 4. Nomenclature and Numbering of the Hepatitis C Virus Carla Kuiken and Peter Simmonds 5. A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates Kathryn J. Rolfe, Tim G. Wreghitt, Graeme J.M. Alexander, and Martin D. Curran 6. Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR1) by In Vitro Translation and Mass Spectrometry Carmen Yea, Melissa Ayers, and Raymond Tellier PART III: HCV REPLICATION IN VITRO 7. Purification and Crystallization of NS5A Domain I of Hepatitis C Virus Joseph Marcotrigiano and Timothy L. Tellinghuisen 8. NS5A Phosphorylation and Hyperphosphorylation Petra Neddermann 9. Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies Stefania Di Marco, Edwin Rydberg, Matthew Bottomley, and Andrea Carfi 10. Structural and Functional Analysis of the HCV p7 Protein Nathalie Saint, Roland Montserret, Christophe Chipot, and François Penin 11. HCV Replicons: Overview and Basic Protocols Volker Lohmann 12. Reverse Transcription PCR Based Sequence Analysis of Hepatitis C Virus RepliconRNA Timothy L. Tellinghuisen and Brett D. Lindenbach 13. Studying HCV RNA Synthesis In Vitro with Replication Complexes Wengang Yang and Mingjun Huang 14. Proteomics Study of the Hepatitis C Virus Replication Complexes Kyungsoo Chang, Tianyi Wang, and Guangxiang Luo 15. Investigation of the Hepatitis C Virus Replication Complex Volker Brass, Rainer Gosert, and Darius Moradpour 16. Regulation of Interferon Regulatory Factor-3-dependent Innate Immunity by the HCV NS3/4A Protease Kui Li 17. Selection and Characterization of Drug Resistant HCV Replicons In Vitro with a Flow CytometryBased Assay Jason M. Robotham, Heather B. Nelson, and Hengli Tang 18. High Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System Weidong Hao and Rohit Duggal 19. Inhibition of HCV Replication by Small Interfering RNA Ratna B. Ray and Tastuo Kanda PART IV: HCV ENTRY AND INFECTION 20. Cellular Receptors and HCV Entry Mike Flint and Donna M. Tscherne 21. Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp) Birke Bartosch and Francois-Loic Cosset 22. Screening of Small-molecule Compounds as Inhibitors of HCV Entry Jian-Ping Yang, Demin Zhou and Flossie Wong-Staal 23. Isolation of the JFH-1 Strain and Development of an HCV Infection System Takaji Wakita 24. Measuring HCV Infectivity Produced in Cell Culture and In Vivo Brett D. Lindenbach 25. Genotype 1a HCV (H77S) Infection System MinKyung Yi and Stanley M. Lemon 26. Full Length Infectious HCV Chimeras Thomas Pietschmann 27. Adaptation of the Hepatitis C Virus to Cell Culture Artur Kaul, Ilka Wörz and Ralf


Titel: Hepatitis C
Untertitel: Methods and Protocols
EAN: 9781588299703
ISBN: 1588299708
Format: Fester Einband
Herausgeber: Humana Press
Genre: Medizin
Anzahl Seiten: 484
Gewicht: 1341g
Größe: H266mm x B198mm x T34mm
Jahr: 2008
Auflage: 2nd ed. 2009

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