This book renders a comprehensive understanding of hematopoietic stem cells (HSCs) from their embryonic development through adult maintenance to aging, in the studies conducted in zebrafish and mammals. Hematopoiesis provides a paradigm for understanding the development, maintenance, regeneration, aging and malignant transformation of mammalian organs. Sitting at the apex of the hematopoiesis hierarchy tree, HSCs orchestrate their proliferation, self-renewal, and differentiation to produce all the blood cell lineages throughout life, which represents the best example for somatic stem cell studies. In this book, key regulatory mechanisms for HSC self-renewal and differentiation are overviewed in an array of fields including epigenetics, metabolism and microenvironment regulation. It also highlights the HSC heterogeneity and clonal dynamics from the recent advanced single-cell technologies. This book elaborates on the research history of HSC studies and reveals how the insights from HSC studies shed light on their clinic application. It presents great value from the bench to the clinic.

Provides a comprehensive understanding of HSCs from embryonic development through adult maintenance to aging Includes the research in epigenetics, metabolism and microenvironment and advanced technologies Offers a good reference for experimental and clinical hematologist, scientists in stem cells and regeneration

Autorentext

Dr. Meng Zhao, a distinguished stem cell biologist and hematologist, is currently serving as a professor at the Zhongshan School of Medicine, Sun Yat-sen University. His research primarily focuses on unraveling the intricate mechanisms governing blood stem cells and developing therapeutic strategies for hematopoietic stem cell transplantation and leukemia treatment. In his laboratory, Dr. Zhao utilizes genetic models and clinical specimens to investigate both the intrinsic cellular control and microenvironmental influence on normal and leukemic stem cells. Noteworthy contributions from Dr. Zhao include identifying megakaryocytes as a hematopoietic stem cell niche, characterizing leukemic stem cells in T-cell acute lymphoblastic leukemia, and discovering amino acid catabolism within hematopoietic stem cells. The primary objective of Dr. Zhao's laboratory is to gain comprehensive insights into the complex metabolic intricacies that govern both normal and leukemic stem cells within the dynamic bone marrow microenvironment.****

Prof. Pengxu Qian obtained his bachelor degree in 2006, and his Ph.D. degree in 2012 from University of Science and Technology of China (USTC). During his postdoctoral training in Prof. Linheng Li's lab at Stowers Institute for Medical Research from 2012 to 2017, Dr. Qian was funded by the American Society of Hematology (ASH) Scholar Award to study the roles of DNA methylation and imprinting genes in hematopoietic stem cells. In Nov 2017, Dr. Qian started his lab as principal investigator in Zhejiang University School of Medicine, to study the roles and mechanisms of epigenetic regulators in normal hematopoiesis and leukemogenesis. The major contributions from Dr. Qian include illustrating the roles and mechanisms of the Dlk1-Gtl2 imprinting locus, the differentially methylated region (DMR) DERARE in the Hoxb cluster, and the m6A reader YTHDF2 in normal hematopoiesis and leukemogenesis. The primary objectives of Dr. Qian's laboratory are to decipher the epigenetic regulatory mechanisms underlying HSC homeostasis and leukemogenesis and to develop novel therapeutic approaches against hematopoietic malignancies.

Inhalt

Chapter 1. Hematopoietic Stem Cell Development in Mammalian Embryos.- Chapter 2. Hematopoietic Stem Cells and Their Bone Marrow Niches.- Chapter 3. Emerging Roles of Epigenetic Regulators in Maintaining Hematopoietic Stem Cell Homeostasis.- Chapter 4. Metabolism in Hematopoiesis and Its Malignancy.- Chapter 5. The Origin of Clonal Hematopoiesis and Its Implication in Human Diseases.- Chapter 6. Ex vivo Expansion and Homing of Human Cord Blood Hematopoietic Stem Cells.- Chapter 7. N6-methyladenosine RNA Modification in Normal and Malignant Hematopoiesis.- Chapter 8. Immune System Influence on Hematopoietic Stem Cells and Leukemia Development.- Chapter 9. Learning from Zebrafish Hematopoiesis.- Chapter 10. Multi-lineage Differentiation from Hematopoietic Stem Cells.- Chapter 11. Gene Editing in Hematopoietic Stem Cells.- Chapter 12. Aging, Causes and Rejuvenation of Hematopoietic Stem Cells