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To aid scientists in a vital subject, this compendium of laboratory protocols reflects the increasing availability of sample prepa... Weiterlesen
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To aid scientists in a vital subject, this compendium of laboratory protocols reflects the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools designed for the analysis of glycosylation.

Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.

Presents a detailed compendium of techniques used routinely in the leading laboratories utilizing current, common technologies, especially those established for proteomics research

Reviews the current practices used for the analysis of the specific single O-GlcNAc found on nuclear and cytoplasmic proteins

Disperses the misconception that only experts can analyze the glycome

Inspires researchers to pursue glycan function through sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools

Includes supplementary material: sn.pub/extras

Section I: Glycan Analysis Subsection A: Released Glycans 1. Analysis of N- and O-Linked Glycans from Glycoproteins Using Maldi-TOF Mass Spectrometry Willy Morelle, Valegh Faid, Frédéric Chirat, and Jean-Claude Michalski 2. Infrared Multiphoton Dissociation (IRMPD) Mass Spectrometry for Structural Elucidation of Oligosaccharides Bensheng Li, Hyun Joo An, Jerry L. Hedrick, and Carlito B. Lebrilla 3. Mass Spectrometry of N-linked Glycans Parastoo Azadi and Christian Heiss 4. Solid-phase Permethylation for Glycomic Analysis Yehia Mechref, Pilsoo Kang, and Milos V. Novotny 5. Method for Investigation of Oligosaccharides Using Phenylhydrazine Derivatization Erika Lattová and Hélène Perreault 6. 2-Dimensional HPLC Separation with Reverse Phase-Nano-LC-MS/MS for the Characterization of Glycan Pools after Labeling with 2-Aminobenzamide Manfred Wuhrer, Carolien A. M. Koeleman, and André M. Deelder 7. Capillary Lectin-Affinity Electrophoresis for Glycan Analysis Kazuaki Kakehi and Mitsuhiro Kinoshita 8. Analysis of Methylated O-Glycan Alditols by CAD-ESI Mass Spectrometry Franz-Georg Hanisch and Stefan Müller 9. High-Throughput and High-Sensitivity Nano-LC/MS and MS/MS for O-Glycan Profiling Hasse Karlsson, Jessica M. Holmén Larsson, Kristina A. Thomsson, Iris Härd, Malin Bäckström, and Gunnar C. Hansson 10. Collision Induced Dissociation Tandem Mass Spectrometry for Structural Elucidation of Glycans Bensheng Li, Hyun Joo An, Jerry L. Hedrick, and Carlito B. Lebrilla 11. The Structural Elucidation of Glycosaminoglycans Vikas Prabhakar, Ishan Capila, and Ram Sasisekharan 12. Labelling Heparan Sulphate Saccharides with Chromophore, Fluorescenceand Mass Tags for HPLC and MS Separations Mark Skidmore, Abdel Atrih, Ed Yates, and Jeremy E. Turnbull 13. Small-Scale Enzymatic Digestion of Glycoproteins and Proteoglycans for Analysis of Oligosaccharides by LC-MS and FACE Gel Electrophoresis Ruby P. Estrella, John M. Whitelock, Rebecca H. Roubin, Nicolle H. Packer, and Niclas G. Karlsson Subsection B: Glycopeptides 14. Enrichment Strategies for Glycopeptides Shigeyasu Ito, Ko Hayama, and Jun Hirabayashi 15. Introductory Glycosylation Analysis Using SDS-PAGE and Peptide Mass Fingerprinting Nicole Wilson, Raina Simpson, and Catherine Cooper-Liddell 16. Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF Bing Gong, Michael Cukan, Richard Fisher, Huijuan Li, Terrance A. Stadheim, and Tillman Gerngross 17. N-Glycosylation Site Analysis of Human Platelet Proteins by Hydrazide Affinity Capturing and LC-MS/MS Urs Lewandrowski and Albert Sickmann 18. LC/MSn For Glycoprotein Analysis: N-Linked Glycosylation Analysis And Peptide Sequencing Of Glycopeptides Nana Kawasaki, Satsuki Itoh, and Teruhide Yamaguchi Subsection C: O-GlcNAcomics 19. Detecting the 'O-GlcNAc-ome'; Detection, Purification, and Analysis of O-GlcNAc Modified Proteins Natasha E. Zachara Subsection D: Glycobioinformatic Tools 20. Preparation of a Glycan Library Using a Variety of Glycosyltrasferases Hiromi Ito, Akihiko Kameyama, Takashi Sato, and Hisashi Narimatsu 21. Data Mining the PDB for Glyco-Related Data Thomas Lütteke and Claus-W. von der Lieth Section II: Carbohydrate-Protein


Titel: Glycomics
Untertitel: Methods and Protocols
EAN: 9781617377693
ISBN: 978-1-61737-769-3
Format: Kartonierter Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 390
Gewicht: 757g
Größe: H260mm x B260mm x T193mm
Jahr: 2010
Untertitel: Englisch
Auflage: Softcover reprint of hardcover 1st ed. 2009

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