Discusses new knowledge gained through a multidisciplinary biophysical approach Describes emerging biophysical techniques in detail Discusses up-to-date applications in glycobiophysics

Autorentext

Yoshiki Yamaguchi, PhD

Synthetic Cellular Chemistry Laboratory

RIKEN Cluster for Pioneering Research

Koichi Kato, PhD
Exploratory Research Center on Life and Living Systems
National Institutes of Natural Sciences

Inhalt

Preface.- Chapter 1: The effect of N-glycans on glycoprotein folding and protein dynamics.- Chapter 2: Synthesis of glycosylated metal complexes for probing carbohydrate-carbohydrate interactions.- Chapter 3: Unraveling of lipid raft organization in cell plasma membranes by single-molecule imaging of ganglioside probes.- Chapter 4: MALDI mass spectrometry imaging of N-linked glycans in tissues.- Chapter 5: Modern-omics technologies for isobaric glycan and glycoconjugate structure determination.- Chapter 6: Synchrotron-radiation vacuum-ultraviolet circular-dichroism spectroscopy for characterizing the structure of saccharides.- Chapter 7: Biophysical analyses for probing glycan-protein interactions.- Chapter 8: Structural aspects of ER glycoprotein quality-control system mediated by glucose tagging.- Chapter 9: Structural basis of protein Asn-glycosylation by oligosaccharyltransferases.- Chapter 10: Visualization of functional structure and kinetic dynamics of cellulases.-Chapter 11: Structure and dynamics of immunoglobulin G glycoproteins.- Chapter 12: Biophysical Approaches to Solve the Structures of the Complex Glycan Shield of Chloroviruses.- Chapter 13: Quantifying weak glycan-protein interactions using a Biolayer Interferometry competition assay: Applications to ECL lectin and X-31 influenza hemagglutinin.