Now fully updated and considerably expanded, Glycoanalysis Protocols, 2nd ed., makes available to all protein scientists, and particularly those working with today's pharmaceuticals, the most advanced and reproducible glycoanalysis techniques currently in use. Developed by highly experienced carbohydrate chemists, biochemists, and physical chemists, these detailed, up-to-date, and proven analytical techniques cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. Special attention has been given to advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics.

Hailed as "indispensable" in its first edition, Glycoanalysis Protocols, 2nd ed., continues with vital, time-tested techniques addressing the needs of both biomedical researchers and protein macromolecular structural chemists. It will well serve all those starting work on the analysis of glycoproteins, as well as more experienced investigators seeking to augment their expertise.

Klappentext

Protein glycosylation is now acknowledged as a major posttranslational modification with significant effects on protein folding, conformation distri­ bution, stability, and activity. The added oligosaccharide chains are large and diverse and have specific recognition motifs important in many aspects of cell interactions and regulation. As such, there is a growing need to communicate the analytical methods of the specialist carbohydrate chemist, biochemist, and physicochemist to protein experts and the pharmaceutical industry. Other areas that come under the influence of the glycosciences are DNA interactions with ubiquitous saccharide-containing antibiotics and antitumor drugs; inhibitors of viral infection; bacterial, mycobacterial, and parasite antigens; glycolipids; glycophosphatidylinositol protein membrane anchors; and (glyco)protein proteoglycan interactions. Compared to the first edition of this book, Glycopro­ tein Analysis in Biomedicine, less emphasis is given to biomedical aspects, but these chapters are still pertinent today. The significant differences in the con­ tent relate to advances in analysis relevant to biotechnology; for example, the production of recombinant glycoproteins and other therapeutics. It must also not be forgotten that the methods here described in Glycoanalysis Protocols are relevant to exploiting the commercial potential of carbohydrates in fields related to agriculture, food, and the domestic and chemical industries. The emphasis of the book remains in bringing the glycosciences into mainstream biochemistry. The analytical methods covered in Glycoanalysis Protocols are the re­ sult of experts translating their life's works into easy-to-follow recipes.

Zusammenfassung
Physicochemical aspects of analysis are emphasized in this text. Coverage includes mass spectrometry of oligosaccharides, NMR, and computer-aided molecular modelling. The necessary analytical techniques are described, allowing for extensive research into the structure and function of glycosylation.

Inhalt
Characterization of Protein Glycosylation.- Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides.- Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography.- Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM).- HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the Carbopac MA1 Column.- HPLC and HPAEC of Oligosaccharides and Glycopeptides.- Analysis of N- and O-Glycans by Pyridylamination.- Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins.- Analysis of Glycosaminoglycans and Proteoglycans.- The Use of Cell and Organ Culture for the Study of Secreted Mucins.- Purification of Gastrointestinal Mucins and Analysis of Their O-Linked Oligosaccharides.- TLC in Structure and Recognition Studies of Glycosphingolipids.- Strategies for Preliminary Characterization of Novel Amphoteric Glycosphingolipids.- Analysis of the Carbohydrate and Lipid Components of Glycosylphosphatidylinos¡tol Structures.- Conformational Analysis of Biantennary Glycopeptides with a Resonance Energy Transfer Technique.