Genome Editing in Animals

This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Genome Editing in Animals: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras Includes supplementary material: sn.pub/extras

Inhalt

Construction and Evaluation of Zinc Finger Nucleases.- Current Overview of TALEN Construction Systems.- CRISPR/Cas9.- Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse.- Genome Editing of Mouse by Cytoplasmic Injection.- Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids.- Genome Editing in Mouse and Rat by Electroporation.- Generation of Knock-in Mouse by Genome Editing.- Genome Editing of Rat.- Gene Targeting in Rabbits: Single-step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids.- Genome Editing of Pig.- Genome Editing of Monkey.- Genome Editing Mediated by Primordial Germ Cell in Chicken.- CRISPR/Cas9-mediated Targeted Knock-in of Exogenous Reporter Genes in Zebrafish.- Genome Editing of Medaka.- A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders using the CRISPR-Cas System.- Genome Editing of Silkworms.- Genome Editing in the Cricket, Gryllus bimaculatus.- Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease.- Genome editing of C. elegans.