This detailed volume examines a wide range of disciplines involving genome assembly and its uses. Covering a diversity of taxa including bacteria, archaea, viruses, animals, plants, and fungi, the book demonstrates the similarities and differences in genome assembly methods and biological differences across those groups, while also describing the latest advances in modern sequencing strategies as well as techniques in the rapidly growing fields of meta-genomics and pan-genomics. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail to ensure successful results in new research.

Authoritative and practical, Genome Assembly serves as an ideal guide for researchers pursuing genome analysis, a highly active area of global biosciences research.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

DNA Sequencing and the Third-Generation Sequencing Revolution.- Methods for Chromosome-Scale Haplotype Reconstruction.- Genome Assembly by a Non-Bioinformatician: Case Study of a Small Predatory Natural Enemy Insect, Nesidiocoris tenuis.- Current Progress in Phased Genome Assembly from Long-Read DNA Sequencing Data.- Laboratory and In-Field Metagenomics for Environmental Monitoring.- Algorithms for Short-Read Viral Haplotype Reconstruction: Challenges, Solutions, and Perspectives.- Applying the Classic Test dN/dS to Detect Selection in Archaea.- Pre-Assembly Read Processing and Quality Control in Fungal Genomes, Exemplified by the Chytrid Batrachochytrium salamandrivorans.- Genome Assembly, Polishing, and Analysis of the Chytrid Batrachochytrium salamandrivorans.- Fusarium Genome Sequencing and Assembly.- Current Approaches in Genome Assembly of Human Fungal Pathogens.- A Draft Genome Assembly for the Neglected Tropical Fungal Disease Pathogen Emarellia grisea and Comparison to Other Pleosporalean Agents Causing Eumycetoma in Humans.- Seaside to Bedside: Assembly in Research for Emerging Human Fungal Pathogen Candida auris.- High-Quality Plant Genomes for and through Pangenomics.- The Cancer Genome in the Long Reads Era.