Aspects of genetic engineering research emphasized in this volume are applications to plants (crop plants and grass, both important for human needs) and new methodologies, such as Tar cloning, which make it much easier to isolate specific regions from complex genomes. Another subject discussed is linear DNA replication of prokaryotes.

Autorentext

Jane K. Setlow, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York.

Inhalt
Nuclear Plasmids of Dictyostelium.- The Translation Initiation Signal in E. Coli and its Control.- Direct Isolation of Specific Chromosomal Regions and Entire Genes by TAR Cloning.- Regulation of Lysine and Threonine Metabolism in Plants.- Genetic Engineering of Plant Chilling Tolerance.- Role of Bacterial Chaperones in DNA Replication.- Transformation of Cereals.- Mechanisms of Initiation of Linear DNA Replication in Prokaryotes.- Diverse Regulatory Mechanisms of Amino acid Biosynthesis in Plants.- Forage and Turf-Grass Biotechnology: Principles, Methods, and Prospects.- Informatics Needs of Plant Molecular Biology.