Gene Therapy of Solid Cancers

This volume provides insight into recent developments on experimental and clinical strategies for cancer gene therapy. Gene Therapy of Solid Cancers: Methods and Protocols guides readers through protocols on gene therapeutic strategies in combination with helpful technical notes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Concise and easy-to-use, Gene Therapy of Solid Cancers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Aptamer Targeting the ERBB2 Receptor Tyrosine Kinase for Applications in Tumor Therapy.- Gene Gun Her2/neu DNA Vaccination: Evaluation Of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model.- MIDGE Technology for the Production of a Fourfold Gene-Modified, Allogenic Cell-Based Vaccine For Cancer Therapy.- Evaluation of Bystander Cell Killing Effects in Suicide Gene Therapy of Cancer: Engineered Thymidylate Kinase (TMPK)/AZT Enzyme-Prodrug Axis.- Oncoleaking: Use of the Pore-forming Clostridium perfringens Enterotoxin (CPE) for Suicide Gene Therapy .- iCaspase 9 Suicide Gene System.- p53-encoding pDNA Purification by Affinity-Chromatography for Cancer Therapy.- A qRT-PCR Method for Determining the Biodistribution Profile of a miR-34a Mimic.- Design and Selection of Antisense Oligonucleotides Targeting Transforming Growth Factor Beta (TGF-b) Isoform mRNAs for the Treatment of Solid Tumors.- RNA Interference for Antimetastatic Therapy.- STAT3 Decoy ODN Therapy for Cancer.- Oncolytic Viral Therapy using Reovirus.- Use of GLV-1h68 for Vaccinia Virotherapy and Monitoring.- Back To The Future: Are Tumor-Targeting Bacteria The Next Generation Cancer Therapy?.- Ethics of Cancer Gene Transfer Clinical Research.- Planning an Academic Clinical Trial.- Production of Plasmid DNA as Pharmaceutical.- Minicircle - Next Generation DNA Vectors for Vaccination.- A Phase 2, Open-Label, Randomized Study of Pexa-Vec (JX-594) Administered by Intratumoral Injection in Patients with Unresectable Primary Hepatocellular Carcinoma.- AntiangiogenicMetargidin Peptide (AMEP) Gene Therapy in Disseminated Melanoma.- Clinical Evaluation of ErbB-targeted CAR T-cells, Following Intra-Cavity Delivery in Patients with ErbB-Expressing Solid Tumors.