Gene Therapy of Cancer

Gene therapy has acquired a huge amount of new pre-clinical and clinical data. This book fully updates the first edition with thorough coverage of established and novel protocols involving both experimental and clinical approaches to cancer gene therapy.

Despite various difficulties, the field of gene therapy, particularly with regard to cancer, has accumulated a tremendous amount of vital pre-clinical and clinical data. "Gene Therapy of Cancer: Methods and Protocols, Second Edition" fully updates the first edition with expert coverage of established and novel protocols involving both experimental and clinical approaches to cancer gene therapy. This state-of-the-art volume contains overviews of new concepts and strategies with chapters on regulatory and ethical issues, developments, problems and possible limitations of design and production of gene therapeutics as well as translational issues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, "Gene Therapy of Cancer: Methods and Protocols, Second Edition" is an ideal guide for all those who wish to explore the fast-paced and critical study of nonviral, viral, experimental and clinical cancer gene therapy.

Describes a broad array of different strategies and methods such as for immunogene therapy, antitumor vaccination, oncolytic virotherapy, and suicide gene therapy Provides state-of-the art protocols and overview chapters for cancer gene therapy, along with step-by-step procedures and instructions for gene therapeutic strategies, including notes and tips for troubleshooting to avoid pitfalls Explores nonviral and viral vectors and relevant gene transfer/delivery systems Includes methods and backgrounds of experimental and clinical gene therapy approaches Covers the important issue of vector design and vector production for clinical applications Features the current regulatory and ethical aspects in translating experimental approaches into clinical application of gene therapy to patients Includes supplementary material: sn.pub/extras

Inhalt
Experimental Approaches in Cancer Gene Therapy: Introduction.- The Development of Gene Therapy: From Monogenic Recessive Disorders to Complex Diseases Such as Cancer.- Vectorology for Cancer Gene Therapy.- Designing Adenoviral Vectors for Tumor-Specific Targeting.- Analysis of HSV Oncolytic Virotherapy in Organotypic Cultures.- Use of Minicircle Plasmids for Gene Therapy.- Transposable Elements as Plasmid-Based Vectors for Long-Term Gene Transfer into Tumors.- Designing Plasmid Vectors.- Development of Bacterial Vectors for Tumor-Targeted Gene Therapy.- Nonviral Transfer Technologies in Cancer Gene Therapy.- Electroporative Gene Transfer.- Gene Gun Delivery Systems for Cancer Vaccine Approaches.- Ultrasound-Mediated Gene Transfection.- Nonviral Jet-Injection Technology for Intratumoral In Vivo Gene Transfer of Naked DNA.- Experimental Studies in Cancer Gene Therapy.- Methods for Constructing and Evaluating Antitumor DNA Vaccines.- Immunity of Lentiviral Vector-Modified Dendritic Cells.- Saporin Suicide Gene Therapy.- Using In Vivo Biopanning for the Development of Radiation-Guided Drug Delivery Systems.- Chemosensitization of Tumor Cells: Inactivation of Nuclear Factor-Kappa B Associated with Chemosensitivity in Melanoma Cells After Combination Treatment with E2F-1 and Doxorubicin.- Induction of Tumor Cell Apoptosis by TRAIL Gene Therapy.- Silencing Epidermal Growth Factor Receptor by RNA Interference in Glioma.- Delivery of Phosphorodiamidate Morpholino Antisense Oligomers in Cancer Cells.- Use of RNA Aptamers for the Modulation of Cancer Cell Signaling.- G-Rich Oligonucleotides for Cancer Treatment.- Clinical Approaches in Cancer Gene Therapy - Requirements for Clinical Gene Therapy Trials.- Regulatory Aspects for Translating Gene Therapy Research into the Clinic.- Ethics of Cancer Gene Transfer Clinical Research.- Virus Production for Clinical Gene Therapy.- Production of Plasmid DNA as Pharmaceutical.- Clinical Protocols in Cancer Gene Therapy.- Gene Immunotherapy for Non-Small Cell Lung Cancer.- Gene Therapy for Antitumor Vaccination.- HSV-tk/IL-2 Gene Therapy for Glioblastoma Multiforme.- Construction and Characterization of an Oncolytic HSV Vector Containing a Fusogenic Glycoprotein and Prodrug Activation for Enhanced Local Tumor Control.- Newcastle Disease Virus: A Promising Vector for Viral Therapy, Immune Therapy, and Gene Therapy of Cancer.- Oncolytic Viral Therapy Using Reovirus.- Design and Testing of Novel Oncolytic Vaccinia Strains.- Tumor-Targeted Salmonella typhimurium Overexpressing Cytosine Deaminase: A Novel, Tumor-Selective Therapy.- Chemoprotection by Transfer of Resistance Genes.- Phase I Clinical Trial of Locoregional Administration of the Oncolytic Adenovirus ONYX-015 in Combination with Mitomycin-C, Doxorubicin, and Cisplatin Chemotherapy in Patients with Advanced Sarcomas.