This second edition is dedicated to new and updated methodological approaches designed to study galectin function. Chapters examine salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Galectins: Methods and Protocols, Second Eidtion aims to be a useful practical guide to researches to help further their study in this field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Galectins: An Ancient Family of Carbohydrate Binding Proteins with Modern Functions.- Cloning, Expression and Purification of Galectins for In Vitro Studies.- Purification of Recombinant Galectins Using Affinity Chromatography Methods.- Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation.- Rapid Detection and Purification of Galectin-3 by the Capture and Release (CaRe) Method.- Introducing 77Se NMR Spectroscopy to Analyzing Galectin-Ligand Interaction.- Evaluation of Galectin Binding by Surface Plasmon Resonance.- Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein.- Examining Galectin Binding Specificity Using Glycan Microarrays.- Mechanism of Mucin Recognition by Lectins - A Thermodynamic Study.- Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis.- Dissecting Context-Specific Galectin Binding Using Glyco-Engineered Cell Libraries.- Method for Identifying GalectinLigands on Lymphocyte Membrane Glycoproteins.- Transformation of Agrocybe Cylindracea Galectin into aGalNAc-specific Lectin.- What Happens if a Human Galectin Enters the Endoplasmic Reticulum?.- Investigation of Galectins in Frozen Tissue and Mammalian Cell Culture Using Confocal Microscopy.- Exploring the Galectin Network by Light and Fluorescence Microscopy.- Molecular Imaging for In Vivo Tracking and Detection of Galectin Binding Partners.- Visualization of Cytosolic Galectin Accumulation Around Damaged Vesicles and Organelles.- Transcytosis of Galectin-3 in Mouse Intestine.- Evaluating the Role of Galectins in Clathrin Independent Endocytosis.- Examination of Galectin-3-Recruitment into Multivesicular Bodies for Exosomal Secretion.- Manipulating Galectin Expression in Zebrafish (Danio Rerio).- Examining Galectin Gene Regulation by Reporter Assays.- Examining the Impact of Galectin-9 on Latent HIV Transcription.- Evaluation of the Role of Galectins in Parasite Immunity.- Evaluationof the Bactericidal Activity of Galectins.- Detection of Phosophatidylserine Exposure on Leukocytes Following Treatment with Human Galectins.- Detection of Reactive Oxygen Species in Human Neutrophils Under Various Conditions of Exposure to Galectin.- Analysis of Galectin-Binding Receptors on B Cells.- Methods for Assessing the Effects of Galectins on Leukocytetrafficking and Clearance.- Examination of the Contributions of Maternal/Placental-Derived Galectin-1 to Pregnancy Outcome.- Method to Study the Role of Galectins in Angiogenesis In Vivo Using the Chick Chorioallantoic Membrane Assay.- Untangling Galectin-Mediated Circuits that Control Hypoxia-Driven Angiogenesis.- Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology.- Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle.- Exploring the Role of Galectins in Cancer: In Vitro and In Vivo Approaches.