Fungal Genomics

This volume details protocols covering nearly all aspects of fungal genomics. New and updated chapters guide the reader through experimental genomics, biotechnologies, and the analysis and processing of data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Fungal Genomics : Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Klappentext
This volume details protocols covering nearly all aspects of fungal genomics. New and updated chapters guide the reader through experimental genomics, biotechnologies, and the analysis and processing of data. Written in the highly successful *Methods in Molecular Biology *series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical,* Fungal Genomics : Methods and Protocols, Second Edition *aims to ensure successful results in the further study of this vital field.

Inhalt

Introduction to the Book: Overview of Fungal Genomics.- Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing.- Purification of Fungal High Molecular Weight Genomic DNA from Environmental Samples.- Genome Sequencing.- Methods for Genomic Characterization and Maintenance of Anaerobic Fungi.- Efficient Extraction Method for High Quality Fungal RNA from Complex Lignocellulosic Substrates.- Isolation of high quality RNA for Metatranscriptome Analysis of Lignocellulose Digestion in the Rumen.- Fungal Transcriptomics.- Mass Spectrometrybased Proteomics.- Mass Spectrometry Based Metabolomics.- Genome Editing - CRISPR/cas9.- Evolutionary Adaptation to Generate Mutants.- Genome Assembly.- Fungal Epigenomics: Detection and Analysis.- Fungal Genome Annotation.- Manual Gene Curation and Functional Annotation.- Evaluating Programs for Predicting Genes and Transcripts with RNA-Seq Support in Fungal Genomes.- Genomic Sequence Variation Analysis by Resequencing.- ChIP-seqAnalysis in Neurospora crass.- Fungal Phylogenomics.- Phylogenetic Analysis of Protein Family.- Parasexual Crossings for Bulk Segregant Analysis in Aspergillus Niger To Facilitate Mutant Identification Via Whole Genome Sequencing.