Formalin-Fixed Paraffin-Embedded Tissues

Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols presents an area of research that intersects with and integrates diverse disciplines. The book's contributions provide practical guidelines to this complex study.

Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.

Includes well-established and tested methods and protocols Provides step-by-step detail essential for reproducible results Contains well-organized themes and key implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Regulatory and Ethical Issues on the Utilization of FFPE Tissues in Research.- Tissue Microarrays: Construction and Uses.- Standardization in Immunohistology.- Multiple Immunofluorescence Labeling of Formalin-fixed Paraffin-embedded (FFPE) Tissue.- Microwaves for Chromogenic-in-situ-hybridization.- Automated Analysis of FISH-stained HER2/neu Samples with Metafer.- Laser Capture Microdissection (LCM) of FFPE Tissue Sections

Bridging the Gap between Microscopy and Molecular Analysis.- Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sections.- Microarray-based CGH and Copy Number Analysis of FFPE Samples.- Microarray Profiling of DNA Extracted from FFPE Tissues using SNP6.0 Affymetrix Platform.- Whole Genome Amplification of DNA Extracted From FFPE Tissues.- Pyrosequencing of DNA Extracted from Formalin-Fixed Paraffin-embedded Tissue.- Analysis of DNA Methylation in FFPE Tissues using the MethyLight Technology.- RT-PCR Gene Expression Profiling of RNA from Paraffin-Embedded Tissues Prepared Using a Range of Different Fixatives and Conditions.- RT-PCR-Based Gene Expression Profiling for Cancer Biomarker Discovery from Fixed Paraffin Embedded Tissues.- microRNA Isolation from Formalin-Fixed Paraffin-Embedded Tissues.- Gene Expression Profiling of RNA Extracted from FFPE tissues: NuGEN Technologies' Whole-Transcriptome Amplification System.- Protein Mass Spectrometry Applications on FFPE Tissue Sections.- An Alternative Fixative to Formalin Fixation for Molecular Applications: the RCL2 â -CS100 Approach