This volume details reliable protocols for the successful implementation of LAMP assays for the detection of the most relevant foodborne pathogens and antibiotic resistance microorganisms. Chapters guides the reader through different types of detection strategies, from the most classical ones to the most recent ones focused on color change or even the implementation of nanomaterials are covered, along with specific applications in point-of care devices and even primer design. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols.

Authoritative and cutting-edge, Food Bourne Pathogen: Loop-mediated Isothermal Amplification Protocols aims to be comprehensive guide for researchers in the field.

Authoritative andcutting-edge, Loop-mediated isothermal amplification: foodborne pathogendetection and characterization aims to provide comprehensive and updated state-of-art methodologies for food analysis.

Contains key notes and implementation advice from the experts Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results

Inhalt

Loop-mediated isothermal amplification (LAMP) a simple method for the detection of food borne pathogens.- Rapid Detection of Salmonella in Frozen Fish and Dry Dog Food.- Design of a Competitive Internal Amplification Control in Multiplex Loop-mediated isothermal amplification.- Detection of thermophilic Campylobacter spp. in poultry meat.- Detection of Shiga Toxin-Producing Escherichia coli with Loop-mediated isothermal amplification (LAMP) and bioluminescent assay in real time (BART) .- LAMP detection of pathogenic Vibrio spp. in seafood and water samples using colori-fluorometric dye.- Loop-mediated isothermal amplification assay (LAMP) for detection of Staphylococcus aureus in meat.- Loop-Mediated Isothermal Amplification for the Detection of Cronobacter sakazakii in Milk Powder.- Rapid Detection of Salmonella ser. Enteritidis by Loop-Mediated Isothermal Amplification (LAMP) method.- One-step Reverse Transcription Loop-Mediated Isothermal Amplification of Norovirus Genogroup I and II in Oysters.- Hepatitis A Virus Detection Using the Loop-Mediated Isothermal Amplification-Based (LAMP) Technique In Foods.- Hepatitis E Virus Detection Using The Loop-Mediated Isothermal Amplification-Based (LAMP) Technique.- Detection of Adenovirus by LAMP.- Detection of spoilage fungi in foods by Loop Mediated Isothermal Amplification.- Detection of aflatoxin producing fungi in food commodities using a neutral red-based visual LAMP-assay.- Fecal contamination assessment through Loop-mediated isothermal amplification of Bacteroidales in food samples.- Implementation of Fluorescent Primers in Loop-mediated Isothermal Multiplex Assays.- Same-day detection of viable Salmonella Enteritidis by phage amplification coupled with loop-mediated isothermal amplification.- Gold Nanoparticle-Based Loop-Mediated Isothermal Amplification for Rapid, Low-Cost, and Simple Detection of Salmonella in Coffee and Cream.- LAMP-on-a-chip in combination with surface-enhanced Raman.