This volume provides both experienced and new microscopists with methods and protocols to perform fluorescence microscopy-based experiments. The book is divided into four parts detailing basic fluorescent microscopy, quantitative methods, imaging living animals, human tissue samples, approaches for imaging at a near-molecular level, and approaches to image analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Fluorescent Microscopy aims to be a useful practical guide to researches to help further their study in this field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Fluorescence Microscopy: A Field Guide for Biologists.- Three-Dimensional Simultaneous Imaging of Nucleic Acids and Proteins During Influenza Virus Infection in Single Cells Using Confocal Microscopy.- Optimizing Long-Term Live Cell Imaging.- Monitoring Transmembrane and Peripheral Membrane Protein Interactions by Förster Resonance Energy Transfer Using Fluorescence Lifetime Imaging Microscopy.- Bimolecular Fluorescence Complementation to Visualize Protein:Protein Interactions in Cells.- Monitoring Cellular Responses to Infection with Fluorescent Biosensors.- Quantifying Endothelial Transcytosis with Total Internal Reflection Fluorescence Microscopy (TIRF).- Measurement of Minute Cellular Forces by Traction Force Microscopy.- Quantitative Immunofluorescent Imaging of Immune Cells in Mucosal Tissues.- Intravital Microscopy Techniques to Image Wound Healing in Mouse Skin.- Quantifiable Intravital Light Sheet Microscopy.- Hydrophobic and Hydrogel-based Methods for Passive Tissue Clearing.- Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos.- Super Resolution Radial Fluctuations (SRRF) Microscopy.- A Practical Guide for STED Imaging.- Single-Molecule Localization Microscopy of Subcellular Protein Distribution in Neurons.- Measuring the Lateral Diffusion of Plasma Membrane Receptors using Raster Image Correlation Spectroscopy.- Nanometer-Scale Molecular Mapping by Super-Resolution Fluorescence Microscopy.- Visualizing and Quantifying Data from Timelapse Imaging Experiments.- Automated Segmentation and Microscopy Image Analysis with Machine Learning.