This volume presents the latest advancements in extracellular vesicles (EV) research, drawing on the expertise of investigators across disciplines and institutions. The chapter in this book cover a wide array of experimental approaches, from foundational isolation and enrichment techniques to cutting-edge analytical tools and therapeutic applications. Chapter topics include tangential flow filtration for large-scale isolation, magnetic bead-based separation, and optimized methods for recovering EVs from diverse biological sources. Some chapter also cover specialized procedures that detail the capture of EV surface proteins, electron tomography-based structural analysis, and innovative strategies to load small RNAs into EVs. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Comprehensive and cutting-edge, Extracellular Vesicles serves as a practical and accessible reference for both new and experienced researchers. Whether working with clinical samples, developing therapeutic EVs, or studying their basic biology, investigators will find detailed protocols accompanied by critical commentary on experimental design, controls, and interpretation.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Extracellular Vesicles: Biology, Functions, and Applications.- A Protocol to Capture the Extracellular Vesicle Surface (Surfaceome) Combining Biotinylation and Mass Spectrometry.- Standardized Protocol for Magnetic Bead-Based Enrichment and Separation of Extracellular Vesicles.- Isolation of CNS-Originating Extracellular Vesicles from Blood and Cell Culture Media.- Isolation of Spontaneously Released Extracellular Vesicles from Human and Mouse Brain.- Methods for Isolating and Characterizing Red Blood Cell Extracellular Vesicles.- Analysis of Urinary Extracellular Vesicles.- From Conditioned Media to Therapeutics: Isolation and Characterization of Mesenchymal Stem Cells Derived Extracellular Vesicles.- Large Scale Isolation of Extracellular Vesicles Using Tangential Flow Filtration Method.- Optimized Buffer Conditions for Storage and Recovery of Extracellular Vesicles.- Nanoparticle Tracking Analysis and Its Application to Study the Role of Small Extracellular Vesicles in Health and Disease.- Innovative Methods to Label, Track, and Quantitate Extracellular Vesicle Uptake.- Protocol for Assessing the Procoagulant and Prothrombotic Potential of Extracellular Vesicles.- Electron Tomography-Based Reconstruction of Extracellular Vesicles in Tissue.- Strategies for Loading miRNA Cargo into Extracellular Vesicles.- Bioinformatics Tools and Databases for Extracellular Vesicles and Particles Research.