This detailed book provides an exhaustive picture of current methods to detect, isolate, and analyze extracellular vesicles (EVs) from diverse sources, now seen as potential disease biomarkers as well as tools for the development of new therapies. Beginning with a section on detection and isolation of EVs, the volume continues with chapters covering different methods to isolate and quantify EVs from specialized tissues/organs and body fluids, methods devoted to analyzing EV components, as well as cutting-edge methods to engineer EVs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Extracellular Vesicles in Diagnosis and Therapy serves as an ideal guide for researchers seeking to learn more about the complexities of cell-to-cell communication.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Aptasensors for Cancerous Exosome Detection.- Detection of Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensor.- Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography.- Isolation and Proteomic Analysis of Mouse Serum Small Extracellular Vesicles for Individual Subject Analysis.- Protocol for Measuring Concentrations of Extracellular Vesicles in Human Blood Plasma with Flow Cytometry.- Targeted Mass Spectrometry-Based Proteomics Method to Quantify Placental Extracellular Vesicles.- Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells.- Isolation and Characterization of Salivary Exosomes for Cancer Biomarker Discovery.- Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance.- Direct Detection of Extracellular Vesicle miRNAs Using a Single-Step RT-qPCR Assay.- Purification and Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesicles.- Lipidomic Analysis of Extracellular Vesicles Isolated from Human Plasma and Serum.- Extracellular Vesicles and their Use as Vehicles of Immunogens.- Isolation and Fluorescent Labeling of Extracellular Vesicles from Cultured Tumor Cells.- Generation, Characterization, and Count of Fluorescent Extracellular Vesicles.- Optimized Protocol for Plasma-Derived Extracellular Vesicles Loading with Synthetic miRNA Mimic Using Electroporation.- Extracellular Vesicle Loading via pH-Gradient Modification.