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Estrogen Receptors

  • Kartonierter Einband
  • 592 Seiten
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This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrog... Weiterlesen
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This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.

The chapter 'Bioinformatics Analysis of Estrogen-Responsive Genes' is open access under a CC BY 4.0 license.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Kathleen (Kathy) Eyster Professor of Physiology and Pharmacology Sanford School of Medicine of the University of South Dakota 605-677-5159

1. The Estrogen Receptors: An Overview from Different PerspectivesKathleen M. Eyster2. Competitive Binding Assay for the G-Protein Coupled Receptor 30 (GPR30) or G-Protein Coupled Estrogen Receptor (GPER)Thomas Thekkumkara, Russell Snyder, and Vardan T. Karamyan3. The Use of Real Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene ExpressionMichelle L. Booze and Kathleen M. Eyster4. Bioinformatics Analysis of Estrogen-Responsive GenesAdam E. Handel 5. Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to EstrogenKwi Hye Koh and Hyunyoung Jeong6. Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory FactorsMeike Wagner, Johannes Jung, Michael Koslowski, Özlem Türeci, Vijay K. Tiwari, and Ugur Sahin7. Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli CellsJing Lin and Zhenmin Lei8. Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast CancerKelly A. Holmes, Gordon D. Brown, and Jason S. Carroll9. RNA-Seq Experiment and Data AnalysisHanquan Liang and Erliang Zeng10. DNA Microarray Analysis of Estrogen-Responsive GenesKathleen M. Eyster11. Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two Dimensional Liquid Chromatography and Tandem Mass SpectrometryEduardo A. Callegari12. Assessment of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate Endometrium, Placenta, and Fetal Adrenal in Response to EstrogenThomas W. Bonagura, Jeffery S. Babischkin, Gerald J. Pepe and Eugene D. Albrecht13. Co-Localization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal MicroscopyAraceli Morales, Raquel Marín, Jorge Marrero-Alonso, Alicia Boto, and Mario Díaz 14. Live Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence MicroscopyKassandra Kisler and Reymundo Dominguez15. In Situ Hybridization of Estrogen Receptors and and GPER in the Human TestisD. Fietz, M. Bergmann, and K. Hartmann16. Purification of Histone Lysine Methyltransferase SMYD2 and Co-Crystallization with a Target Peptide from Estrogen Receptor aYuanyuan Jiang, Joshua Holcomb, Nicholas Spellmon, and Zhe Yang17. Gold Nanoparticle-Based Förster Resonance Energy Transfer (FRET) Analysis of Estrogen Receptor: DNA InteractionXiaodi Su, Khin Moh Moh Aung, Steven Lukman, and Bin Liu 18. Analysis of Interaction of Estradiol with Estrogen Receptor by NMR SpectroscopyM.K. Thakur and V. Paramanik19. Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction between the Estrogen Receptor Alpha and Co-Activators: The Case of CalmodulinEmeric Miclet, Sandrine Bourgoin-Voillard, Cillian Byrne, and Yves Jacquot20. Surface Plasmon Resonance Study of Cooperative Interactions of Estrogen Receptor and Specificity Protein 1 with Composite DNA ElementsXiaodi Su and Hong Yan Song21. Tips and Tricks for Successful Application of Statistical Methods to Biological DataEvelyn Schlenker22. The Synonymous Ala87 Mutation of Estrogen Receptor Alpha Modifies Transcriptional Activation through Both ERE and AP1 SitesTamara Fernández-Calero, Gilles Flouriot, and Mónica Marín<23. Selective Estrogen Receptor Modulators and the Tissue-Selective Estrogen Complex: Analysis of Cell Type-Specific Effects using In Vivo Imaging of a Reporter Mouse ModelSara Della Torre and Paolo Ciana24. Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish ModelDaniel A. Gorelick, Caroline Lucia Pinto, Ruixin Hao, and Maria Bondesson25. Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo CultureNatalija Eigeliene, Risto Erkkola, and Pirkko Härkönen26. Estrogen Receptor Agonists and Antagonists in the Yeast Estrogen BioassaySi Wang and Toine F. H. Bovee27. Silencing Estrogen Receptor- with siRNA in the Intact Rodent BrainAna C. Ribeiro, Anders Ågmo, Sergei Musatov, and Donald W. Pfaff28. Silencing Estrogen Receptor- with siRNA in Cultured CellsRen-Jun Hsu and Jui-Ming Liu29. Experimental Procedures for Demonstration of MicroRNA Mediated Enhancement of Functional Neuroprotective Effects of Estrogen Receptor AgonistsMrinmay Chakrabarti and Swapan K. Ray 30. Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer CellsAnne Katchy and Cecilia Williams31. Estradiol-Induced Transcriptional Regulation of Long Non-Coding RNA, HOTAIRArunoday Bhan and Subhrangsu S. Mandal32. Detection and Functional Analysis of Estrogen Receptor Phosphorylated at Serine 216 in Mouse NeutrophilsSawako Shindo, Rick Moore, and Masahiko Negishi33. Estrogen Receptor- Knockout MicePer Antonson, Patricia Humire, and Jan-Åke Gustafsson34. Detection of Endogenous Selective Estrogen Receptor Modulators such as 27-HydroxycholesterolErik R. Nelson35. Phytoestrogens Activate the Estrogen Receptor in HepG2 CellsLynne A. Kelly36. Detection of the Phosphorylation of the Estrogen Receptor a as an Outcome of GPR30 ActivationSara Clark, Kevin Pollard , Jennifer Rainville, and Nandini Vasudevan37. GPER Mediates Non-Genomic Effects of EstrogenMarco Pupo, Marcello Maggiolini, and Anna Maria Musti38. GPER/GPR30 Knockout Mice: Effects of GPER on MetabolismGeetanjali Sharma and Eric R. Prossnitz39. Analysis of G-Protein Coupled Receptor 30 (GPR30) on Endothelial Inflammation Subhadeep Chakrabarti and Sandra T. Davidge40. Atherosclerosis and Vascular Biologic Responses to Estrogens: Histologic, Immunohistochemical, Biochemical, and Molecular MethodsThomas C. Register, Susan E. Appt, and Thomas B. Clarkson 41. Assessing Direct Vascular Actions of EstrogensDouglas S. Martin, and Kathleen M. Eyster42. Molecular Design, Synthesis, and Evaluation of SNIPER(ER) that Induces Proteasomal Degradation of ERKeiichiro Okuhira, Yosuke Demizu, Takayuki Hattori, Nobumichi Ohoka, Norihito {Itoh, 2010 #4}Shibata, Masaaki Kurihara, and Mikihiko Naito43. Regulation of Activation Induced Deaminase (AID) by EstrogenSiim Pauklin


Titel: Estrogen Receptors
Untertitel: Methods and Protocols
EAN: 9781493950065
ISBN: 1493950061
Format: Kartonierter Einband
Herausgeber: Springer New York
Anzahl Seiten: 592
Gewicht: 1098g
Größe: H254mm x B178mm x T31mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2016

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