Environmental Genomics

Environmental genomics seeks to predict how an organism or organisms will respond, at the genetic level, to changes in their external environment. These genome responses are diverse and, as a result, environmental genomics must integrate molecular biology, physiology, toxicology, ecology, systems biology, epidemiology and population genetics into an interdisciplinary research program. Environmental Genomics serves as a manual for an environmental scientist who wishes to embrace genomics in an effort to answer environmental questions. The volume is divided into the sections: gene expression profiling, whole genome and chromosome mutation detection, and methods to assay genome diversity and polymorphisms within a particular environment. The studies presented will reduce the uncertainties associated with environmental risk assessment and provide a systematic framework for determining environmental impact and ensuring human health and the sustainability of natural populations. Environmental Genomics should aid researchers looking to reduce the uncertainties associated with environmental risk assessment and provide a systematic framework for determining environmental impact and ensuring human health and the sustainability of natural populations.

Concise protocols to conduct genomic and molecular biology analysis Trouble shooting guide

Inhalt
Gene Expression Profiling.- High Throughput Whole Mount In Situ Hybridization of Zebrafish Embryos for Analysis of Tissue-Specific Gene Expression Changes After Environmental Perturbation.- Fluorescent RNA Arbitrarily Primed Polymerase Chain Reaction.- Isolation of O$3$-Response Genes from Arabidopsis thaliana Using cDNA Macroarray.- Use of cDNA Macroarrays and Gene Profiling for Detection of Effects of Environmental Toxicants.- Constructing and Screening a cDNA Library.- Comparative Molecular Physiological Genomics.- Proteomic Analysis of Neuroendocrine Peptidergic System Disruption Using the AtT20 Pituitary Cell Line as a Model.- Proteomics-Based Method for Risk Assessment of Peroxisome Proliferating Pollutants in the Marine Environment.- Environmental Metabolomics Using 9 H-NMR Spectroscopy.- Detection of Whole Genome Mutation.- Restriction Landmark Genome Scanning for the Detection of Mutations.- Use of the Comet Assay in Environmental Toxicology.- The Micronucleus Assay Determination of Chromosomal Level DNA Damage.- Fluorescence In Situ Hybridization for the Detection of Chromosome Aberrations and Aneuploidy Induced by Environmental Toxicants.- Laboratory Methods for the Detection of Chromosomal Structural Aberrations in Human and Mouse Sperm by Fluorescence In Situ Hybridization.- Determination of Species Diversity.- Assembling DNA Barcodes.- Application of Suppressive Subtractive Hybridization to Uncover the Metagenomic Diversity of Environmental Samples.- 16S rRNA Targeted DGGE Fingerprinting of Microbial Communities.- An Emulsion Polymerase Chain ReactionBased Method for Molecular Haplotyping.