This detailed volume highlights the heterogeneity and plasticity of enteric glial cells and explores their contributions to both GI homeostasis and disease pathophysiology. Through an interdisciplinary approach, the book brings together expertise from neurobiology, immunology, physiology, pharmacology, and pathology to provide a comprehensive perspective on the enteric nervous system and its glial components. It explores electrophysiological techniques, the relationship between enteric glia and the gut microbiota, advances in immunohistochemistry, the influence of the gut-brain axis on neurodegenerative diseases and mood disorders, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Enteric Glial Cells: Methods and Protocols serves as an ideal guide for understanding enteric glia but also for stimulating new research avenues that could lead to breakthroughs in treating a range of gastrointestinal and neurological disorders.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Introducing Enteric Glial Cells.- Immunohistochemical Localization and Anatomical Distribution of Enteric Glia Cells in Enteric Nervous System.- Immunohistochemical Staining Protocol of Enteric Glial Cells in Myenteric Plexi.- Rodent Enteric Glia Culture.- Isolation of Distinct Populations of Enteric Glia from the Muscularis Externa and Mucosa of the Mouse Intestine.- Isolation and Dissection Procedures for EGC Isolation by Human Mucosal Biopsies.- Differentiating Enteric Glial Cells Within Intestinal Organoid Derived from Human Pluripotent Stem Cells.- Transgenic Mouse Models to Study Enteric Glia.- Enteric Glial Cell Culture to Mimic In Vivo Obesity and Neurodegenerative Disorders.- Fluorescence-Activated Cell Sorting of Primary Enteric Glia.- Methods to Isolate Enteric Glia from hPSC-Derived Enteric Ganglioids.- Calcium Imaging Studies in Enteric Glia.- In Vitro Manipulation of Gene Expression in Enteric Glial Cells.- In Vitro Study of Microbiota Influence on Enteric Glial Cell Physiology.- Intracerebral Transplantation of Enteric Glia.