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Endocannabinoid Signaling

  • Fester Einband
  • 285 Seiten
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This volume encompasses all major methodologies to interrogate endocannabinoid systems (ECS) and endocannabinoids (eCBs) signaling... Weiterlesen
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This volume encompasses all major methodologies to interrogate endocannabinoid systems (ECS) and endocannabinoids (eCBs) signaling. With increasing interest towards the manifold activities of eCBs, this book discusses the chemical, biochemical, and molecular biological assays, and activity of distinct elements of the ECS. These include membrane, nuclear receptors, biosynthetic and hydrolytic enzymes, and membrane transporters and oxidative enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocols is a valuable resource and will help chemists, drug designers, biochemists, molecular biologists, cell biologists, pharmacologists, and (electro) physiologists navigate the mare magnum of endocannabinoid research.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implelementation advice from the experts

1. Need for Methods to Investigate Endocannabinoid Signaling Mauro Maccarrone 2. Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological TissuesLaura Bindila and Beat Lutz3. Determination of 2-Arachidonoylglycerol by µSPE-LC-MS/MSNatalia Battista and Manuel Sergi 4. Analysis of Omega-3 Fatty Acid Derived N-acylethanolamines in Biological Matrices Renger F. Witkamp and Michiel G.J. Balvers 5. Assay of CB1 Receptor Binding Valeria Catani and Valeria Gasperi 6. The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells Maria Grazia Cascio, Pietro Marini, and Roger G. Pertwee 7. Assay of TRPV1 Receptor Signaling< Aniello Schiano Moriello and Luciano De Petrocellis 8. A Functional Assay for GPR55: Envision Protocols Sharon Anavi-Goffer and Ruth A. Ross 9. The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells Pietri Marini, Maria Grazia Cascio, and Roger G. Pertwee 10. Assay of GTPS Binding in Autoradiography Marina Gabaglio, Pamela Prini, Erica Zamberletti, Tiziana Rubino, and Daniela Parolaro 11. Protocol to Study -Arrestin Recruitment by CB1 and CB2 Cannabinoid Receptors Marjolein Soethoudt, Noortje van Gils, Mario van der Stelt, and Laura H. Heitman 12. Assay of NAT Activity Toru Uyama and Matsuo Ueda 13. Assay of NAPE-PLD Activity Filomena Fezza, Nicolina Mastrangelo, and Mauro Maccarrone 14. Assay of FAAH Activity Monica Bari, Monica Feole, and Mauro Maccarrone 15. Assay of NAAA Activity Kazuhito Tsuboi and Natsuo Ueda 16. Assay of DAGL/ Activity Tiziana Bisogno 17. Assay of Monoacylglycerol Lipase Activity Kwang-Mook Jung and Daniele Piomelli 18. A Sensitive and Versatile Fluorescent Activity Assay for ABHD6 Juha R. Savinainen, Dina Navia-Paldanius, and Jarmo T. Laitinen 19. A Sensitive and Versatile Fluorescent Activity Assay for ABHD12 Juha R. Savinainen, Dina Navia-Paldanius, and Jarmo T. Laitinen 20. Assay of Endocannabinoid Uptake Mark Ray, Simon Nicolussi, Andreas Chicca, and Jürg Gertsch 21. Assay of Endocannabinoid Oxidation by Cyclooxygenase-2 Shalley N. Kudalkar, Philip J. Kingsley, and Lawrence J. Marnett 22. Oxygenation of Anandamide by Lipoxygenases Guus van Zadelhoff and Mario van der Stelt 23. Assay of Endocannabinoid Oxidation by Cytochrome P450 Natasha T. Snider, Vyvyca J. Walker, and Paul F. Hollenberg 24. Assessing Gene Expression of the Endocannabinoid System Mariangela Pucci and Claudio D'Addario 25. Western Blotting of the Endocannabinoid System Jim Wager-Miller and Ken Kackie 26. Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells Jim Wager-Miller and Ken Mackie 27. Measuring ECS Interaction with Biomembranes Clotilde B. Angelucci, Annalaura Sabatucci, and Enrico Dainese 28. Visualization of Endocannabinoids in the Cell Sergio Oddi, Antonio Totaro, and Mauro Maccarrone


Titel: Endocannabinoid Signaling
Untertitel: Methods and Protocols
EAN: 9781493935376
ISBN: 978-1-4939-3537-6
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Medizin
Anzahl Seiten: 285
Gewicht: g
Größe: H17mm x B259mm x T183mm
Jahr: 2016
Auflage: 1st ed. 2016

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