This book reviews the fundamentals of electric cell-substrate impedance sensing, and recent progress which permits high-throughput automated analyses. Coverage focuses on applications in cell based investigations, particularly in the area of cancer biology.

Cell based impedance sensing is becoming a new biophysical and cellular technology in cell based analyses. The technology has been used in investigation of cellular growth and death, cell adhesion and migration, cell invasion and cell-cell interactions, cell toxicity, angiogenesis, cell permeability, signal transduction and cellular behaviour under flow conditions. It is a probe free, highly sensitive, and versatile technology platform. Recent development in the technology has also allowed high throughput, automated analyses. It has been widely explored in chemistry, toxicity, cell biology, cancer biology, and other areas of chemistry, medicinal chemistry, life and medical science. Written by experts in the area of cell impedance sensing, including the Nobel Laureate Dr Ivar Giaever, this books covers the background of electric cell-substrate impedance sensing, their applications in cell based investigations, particularly in the area of cancer biology. This book is the first on thistechnology platform and will be a highly useful reference for molecular and cell biologists, cancer biologists, chemists and biochemists, clinical researchers who work in the areas of cell biology, molecular biology, toxicology, pharmaceutical industry, life science and medical research.

The first book devoted to cell based impedance sensing Written by experts in this area Highly informative colour figures Protocols and methods

Inhalt

Preface.-1 Electric Cell-substrate Impedance Sensing Concept to Commercialisation.-2 Protein Kinase C Isoforms in the Formation of Focal Adhesion Complexes: Investigated by Cell Impedance.-3 ECIS as a tool in the study of metastasis suppressor genes: Epithelial Protein Lost In Neoplasm (EPLIN).-4 Electrical Cell-Substrate Impedance Sensing for Measuring Cellular Transformation, Migration, Invasion, and Anticancer Compound Screening.-5 Epithelial-mesenchymal transition and the use of ECIS.-6 Cell Growth and Cell Death Studied by Electric Cell-Substrate Impedance Sensing.-7 Tight Junctions in Cancer Metastasis and their investigation using ECIS (Electric Cell-substrate Impedance Sensing).-8 Epithelial wound healing and the effects of cytokines investigated by ECIS.-9 Tumour-endothelial and tumour-mesothelial interactions investigated by impedance sensing based cell analyses.-10 Application of Electric Cell-Substrate Impedance Sensing in evaluation of traditional medicine on the cellular functions of gastric and colorecctal cancer cells.-11 Electric cell-substrate impedance sensing as a screening tool for wound healing agents.-12 ECIS, cellular adhesion and migration in keratinocytes.-13 Current and future application of ECIS models to study bone metastasis.Index.