Ebolaviruses

This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Ebolaviruses: Methods and Protocols serves as a guide to the next generations of filovirus researchers and also helps to bring experts from other areas into the filovirus research arena.

Includes cutting-edge techniques for the study of ebolaviruses Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts Includes supplementary material: sn.pub/extras Includes supplementary material: sn.pub/extras

Inhalt
Marburg and Ebola Viruses: A Look Back and Lessons for the Future.- 40 Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent through the Development and Application of New Technologies.- Ebolavirus: An Overview of Molecular and Clinical Pathogenesis.- Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms.- Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins.- Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems.- Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation.- Modeling Ebolavirus Budding with Virus-Like Particles.- Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Virus-Like Particle Assays.- Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins.- Non-Radioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA.- A Semi-Automated High Throughput Microtitration Assay for Filoviruses.- Generation of Recombinant Ebola Viruses Using Reverse Genetics.- Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals.- Live-Cell Imaging of Filoviruses.- Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds.- Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence.- Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells.- Electron Microscopy of Ebola Virus-Infected Cells.- Validating the Inactivation Effectiveness of Chemicals on Ebola Virus.- Visualizing Ebolavirus Particles Using Single-Particle Interferometric Reflectance Imaging Sensor (SP-IRIS).- Assessing Antiviral Countermeasures Using Mouse Models of Ebolavirus Infection.- Evaluation of Ebola Virus Countermeasures in Guinea Pigs.- Evaluation of Medical Countermeasures against Ebolaviruses in Non-Human Primate Models.- Quantification of Filovirus Glycoprotein-Specific Antibodies.- Monitoring Innate Immune Gene Responses in the Hamster Model of Ebola Virus Disease by RT-PCR.- Real-Time and End Point PCR Diagnostics for Ebola Virus.- Production of Antigens for ELISA.- ELISA Methods for the Detection of Ebolavirus Infection.- Ebola Virus Field Sample Collection. <p