This book is the first comprehensive review of drebrin, which plays pivotal roles in various cellular events, via forming unique actin cytoskeletons, including synapse formation and in synaptic function. Particularly the loss of drebrin from dendritic spines is used as a marker of dementia in neurological disorders such as Alzheimer's disease. Since drebrin was first identified by our group in 1985, many studies of drebrin have been done in various fields, including not only molecular biology, biophysics, cell biology, neuroscience, clinical studies, spermatogenesis, immunology, and cancer metastasis, but others as well. The structure of this book facilitates the understanding of the whole picture of studies on drebrin. The volume begins with a general introduction to drebrin, and then the chapters in the second part provide the basic knowledge for further understanding. The third part examines its function in the nervous system, and the fourth part discusses its function in the non-nervous system. This work will appeal to researchers who are interested in cytoskeletal dynamics at membrane-cytoskeletal interface as well as the number of them who use drebrin as a tool, such as a marker of synaptic function or a disease marker. This volume is kept as concise as possible in order to be understood by readers in diverse scientific disciplines.

Offers the first comprehensive review of drebrin from its molecular structure and function to the physiological and pathological roles edited by the discoverer of drebrin Presents a multidisciplinary review of drebrin including that of neuroscience, cancer science, immunology, and cell biology in various tissues Provides the most up-to-date knowledge and intriguing hypotheses related to drebrin and drebrin-decorated F-actin by authoritative original authors of key papers Includes supplementary material: sn.pub/extras Includes supplementary material: sn.pub/extras

Autorentext

Tomoaki Shirao, MD, PhD
Department of Neurobiology and Behavior
Gunma University Graduate School of MedicineGunma, Japan

Yuko Sekino, PhD
Division of Pharmacology
National Institute of Health SciencesTokyo, Japan

Inhalt

Preface.- Part 1 History of drebrin discovery as an general introduction.- Chapter1. Introduction.- Part 2 Basic information about drebrin.- Chapter2. Molecular biology of drebrin.- Chapter3. Biochemistry of drebrin and its binding to actin filaments.- Chapter4. Phosphorylation and molecular structure.- Chapter5. Biophysics of drebrin binding actin filament.- Chapter6. Cell shape change by drebrin.- Part 3 Drebrin in Nervous system.- Chapter7. Localization of drebrin in nervous system: LM study.- Chapter8. Localization of drebrin in neurons: EM study.- Chapter9. Drebrin and spine formation.- Chapter10. Activity-dependent change of drebrin localization.- Chapter11. Drebrin in neurogenesis and neuronal migration.- Chapter12. Drebrin in glia.- Part 4 Drebrin in Dementia.- Chapter13. Drebrin in mild cognitive impairment and Alzheimer's disease.- Chapter14. Amyloid beta and drebrin.- Part 5 Drebrin-binding proteins other than actin filament.- Chapter15. Introduction.- Chapter16. Homer and drebrin.- Chapter17. Spiker mediates drebrin-dependent spine formation.- Part 6 Drebrin in non-nervous system.- Chapter18. Drebrin in immune system.- Chapter19. Drebrin in spermatogenesis.- Chapter20. Drebrin in polarized cells.- Chapter21. Drebrin-dependent stabilization of nectin for maintaining endothelial integrity.- Chapter22. Drebrin in miogenesis.- Part 7 Assorted data relating to drebrin.- Chapter23. Nucleotide and animo acid sequencing of drebrins.- Chapter24. Available antibodies.- Chapter25. Available knockout mice.- Chapter26. How to measure drebrin isoform by ELISA.- Chapter27. Measurement of drebrin accumulation in the spine.- Chapter28. FRAP analysis of drebrin in the spine.