DNA Nanotechnology

Nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the behavior and function of DNA. This book is a guide to scientists of all backgrounds and aims to ignite interest and activity in this young research field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Synthesis and Characterization of Self-assembled DNA Nanostructures .- Protocols for Self-assembly and Imaging of DNA Nanostructures .- Self-Assembly of Metal-DNA Triangles and DNA Nanotubes with Synthetic Junctions .- DNA-Templated Pd Conductive Metallic Nanowires .- A Method to Map Spatiotemporal pH Changes Inside Living Cells using a pH Triggered DNA Nanoswitch .- Control of Helical Handedness in DNA and PNA Nanostructures .- G-quartet, G-quadruplex, and G-wire Regulated by Chemical Stimuli .- Preparation and Atomic Force Microscopy of Quadruplex DNA .- Synthesis of Long DNA-based Nanowires .- G-wire Synthesis and Modification with Gold-nanoparticle .- Preparation of DNA Nanostructures with Repetitive Binding Motifs by Rolling Circle Amplification .- Controlled Confinement of DNA at the Nanoscale: Nanofabrication and Surface Bio-functionalization .- Templated Assembly of DNA Origami Gold Nanoparticle Arrays on Lithographically Patterned Surfaces .- DNA-modified Single Crystal and Nanoporous Silicon .- The Atomic Force Microscopy as a Lithographic Tool: Nanografting of DNA Nanostructures for Biosensing Applications .- Trapping and Immobilization of DNA Molecules Between Nanoelectrodes .- DNA Contour Length Measurements as a Tool for the Structural Analysis of DNA and Nucleoprotein Complexes .- DNA Molecular Handles for Single Molecule Protein Folding Studies by Optical Tweezers .- Optimal Practices for Surface-tethered Single Molecule Total Internal Reflection Fluorescence Resonance Energy Transfer Analysis .- Engineering Mononucleosomes for Single-Pair FRET Experiments .- Measuring DNA-Protein Binding Affinity on a Single Molecule Using Optical Tweezers .- Modeling Nanopores for Sequencing DNA