DNA and RNA Profiling in Human Blood

This book contains both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. It features lists of the necessary materials and reagents as well as tips on troubleshooting and avoiding known pitfalls.

Blood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes. In DNA and RNA Profiling in Human Blood: Methods and Protocols , leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes. Written in the highly successful Methods in Molecular Biology ™ series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA and RNA Profiling in Human Blood: Methods and Protocols is an ideal guide to the molecular profiling approaches that have opened up this broad field of research and have shown great promise in the further identifying of disease markers in blood.

Details numerous techniques to perform blood group genotyping, covering prenatal blood grouping from maternal plasma to multiplex HLA genotyping Presents not only established but also cutting-edge developed protocols for complex and high-throughput DNA and RNA profiling Covers low- to high-throughput methods for SNP typing such as pyrosequencing or microbeads Includes protocols for RNA profiling by modern PCR and bead chip approaches Provides an overview of up-to-date DNA and RNA profiling technologies with plenty of methodological details to help you to establish protocols in your own lab

Inhalt
DNA Profiling for Blood Cell Antigens.- PCRELISA for High-Throughput Blood Group Genotyping.- Single Base Extension in Multiplex Blood Group Genotyping.- Real-Time PCR Assays for High-Throughput Blood Group Genotyping.- Real-Time PCR Assays for High-Throughput Human Platelet Antigen Typing.- Multiplex ABO Genotyping by Minisequencing.- Multiplex Genotyping for Thrombophilia-Associated SNPs by Universal Bead Arrays.- Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance.- Multiplex HLA-Typing by Pyrosequencing.- High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Approach.- Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeads.- The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping.- RNA Profiling in Blood Cells.- Nanoliter High-Throughput PCR for DNA and RNA Profiling.- RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis.- RNA Profiling in Peripheral Blood Cells by Fluorescent Differential Display PCR.- cDNA Amplification by SMART-PCR and Suppression Subtractive Hybridization (SSH)-PCR.- Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGE).- Genome-Wide Platelet RNA Profiling in Clinical Samples.- PCR-Based Amplification of Platelet mRNA Sequences Obtained From Small-Scale Platelet Samples.- MicroRNA Profiling of Megakaryocytes.- Serial Analysis of Gene Expression Adapted for Downsized Extracts (SAGE/SADE) Analysis in Reticulocytes.- Real-Time PCR Analysis for Blood Cell Lineage Specific Markers.- Monitoring the Immune Response Using Real-Time PCR.