This overview of the state of the art in non-amplified DNA detection methods is an ideal introduction to the subject. It explains the principles of the detection methods currently available, as well as highlighting their respective advantages and limitations.

This book offers an overview of state-of-the-art in non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in this field.

The first book dedicated to amplification-free DNA analyses Presents the most cutting-edge methods and probes to analyze DNA Features DNA-sequencing techniques that offer advantages for timely results, enhanced sensitivity and reduced costs Each chapter offers an introduction with the general principles of the method(s) under discussion Self-contained chapters provide easy access and cater for the specific interests of individual readers

Autorentext

Prof. Giuseppe Spoto, University of Catania, Catania, Italy

Prof. Roberto Corradini, University of Parma, Parma, Italy

Klappentext

This book offers a state-of-the-art overview on non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. The importance of non-amplified DNA sequencing technologies will be also discussed.

Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in the field.

Inhalt
Preface.- List of contributors.- Part I DNA analysis: current issues.- 1 Genetic analyses in health laboratories: current status and expectations.- 2 DNA analyses in food safety and quality: current status and expectations.- Part II Advanced materials for ultrasensitive analysis.- 3 Engineered nanostructures for ultrasensitive DNA detection.- 4 Advanced molecular probes for sequence-specific DNA recognition.- 5 Charged conjugated polymers.- 6 Optical detection of non-amplified genomic DNA.- 7 Electrochemical detection of DNA using nanomaterials-based sensors.- 8 Piezoelectric sensing for sensitive detection of DNA.- 9 Surface plasmon resonance-based methods.- 10 Parallel optical and electrochemical DNA detection.- 11 Recent advances in DNA sequencing technologies.- Subject Index.