Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the ...
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Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second Edition presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful Methods in Molecular Biology™ series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Dendritic Cell Protocols, Second Edition aims to become a bench-side handbook for both beginners and experts in the field of DC research and a long-term reference for some of the most popular methods put forward by those who lead the field.
Provides step-by-step procedures for DC isolation from rodent and human tissue, as well as easy in vitro generation of equivalent cell types Explores diverse assays to measure DC function in the context of tolerance, autoimmune disease, allergy, and immunity to viral and bacterial infection All assays used currently by leading experts in the field for high impact studies Preluded by a digestible introduction to DC function and development, covering both humans and rodents Includes techniques that are cutting edge and state-of-the-art in the field of DC biology Contains a comprehensive list of models to test the effect that your favourite gene or immunological model has on DC development or function Helps readers to generate a particular specialized DC subtype in vitro saving time with greater focus on a biologically relevant system Examines isolating DC from almost all organs of the mouse Aids in the identification of the myriad subtypes in human and mice for further study Provides a standardized model for the use of human DCs in clinical trials Includes supplementary material: sn.pub/extras Inhalt Part I: Reviews 1. Review of Human DC subtypes Xinsheng Ju, Georgina Clark, and Derek N.J. Hart 2. Review of Murine Dendritic Cells: Types, Location, and Development Tewfik Miloud, Günter J. Hämmerling, and Natalio Garbi Part II: Human DC Protocols 3. Isolation of Human Blood DC Subtypes Andrew J. Kassianos, Sarah L. Jongbloed, Derek N.J. Hart, and Kristen J. Radford 4. Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin Marein A.W.P. de Jong, Lot de Witte, and Teunis B.H. Geijtenbeek 5. Isolation and in vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells Remko Schotte, Heike Schmidlin, Maho Nagasawa, Wendy Dontje, Julien J. Karrich, Christel Uittenbogaart, Hergen Spits, and Bianca Blom 6. In vivo Modulation of Gene Expression by Lentiviral Transduction in 'Human Immune System' Rag2-/-gc-/- Mice Anja U. van Lent, Mireille Centlivre, Maho Nagasawa, Julien J. Karrich, Stephan Pouw, Kees Weijer, Hergen Spits, Bianca Blom, and Nicolas Legrand 7. Generation of Stable Th1/CTL-, Th2- and Th17-inducing Human Dendritic Cells Pawel Kalinski, Eva Wieckowski, Ravikumar Muthuswamy, and Esther de Jong 8. Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation Heth R. Turnquist, Ryan Fischer, and Angus W. Thomson 9. Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines Michael Erdmann and Beatrice Schuler-Thurner Part III: Murine Dendritic Cell Methods 10. Generation of Mouse CD8+, CD8, and Plasmacytoid Dendritic Cells Equivalents in vitro Using flt3 Ligand Shalin H. Naik, Meredith O'Keeffe, Anna Proietto, Hubertus Hochrein, KenShortman, and Li Wu 11. Generation of Large Numbers of pro-DCs and pre-DCs in vitro Shalin H. Naik 12. The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSF Meredith O'Keeffe, Ben Fancke, and Hubertus Hochrein 13. Isolation of Common Dendritic Cell Progenitor (CDP) from Mouse Bone Marrow Nobuyuki Onai, Markus G. Manz, and Michael A. Schmid 14. The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow Cytometry David Vremec 15. Isolation of Cutaneous Dendritic Cells Julie Helft and Miriam Merad 16. Isolation of Skin Dendritic Cells from Mouse and Man Patrizia Stoitzner, Nikolaus Romani, Alexander D. McLellan, Christoph H. Tripp, and Susanne Ebner 17. Identification and Isolation of Rodent Respiratory Tract Dendritic Cells Philip A. Stumbles, Deborah H. Strickland, Mathew E. Wikstrom, Jennifer A. Thomas, Christophe von Garnier, and Patrick G. Holt 18. Isolation of Mouse Thymic Dendritic Cell Precursors Li Wu and Angela D'Amico 19. Isolation of Rat Intestinal Lymph DC Simon Milling and Gordon MacPherson 20. Mouse Models of Viral Infection: Influenza Infection in the Lung Adele M. Mount and Gabrielle T. Belz 21. DCs in Mouse Models of Intracellular Bacterial Infection Michael Neuenhahn, Matthias Schiemann, and Dirk H. Busch 22. Studying the Function of Dendritic Cells in Mouse Models of Asthma Philippe Pouliot, Monique A. Willart, Hamida Hammad, and Bart N. Lambrecht 23. Direct ex vivo Activation of T cells for Analysis of Dendritic Cell Antigen Presentation Gabrielle T. Belz 24. Methods to Study Pulmonary Dendritic Cell Migration Claudia Jakubzick and