In Cytotoxic T-Cells: Methods and Protocols , leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cytotoxic T-Cells: Methods and Protocols seeks to aid scientists in the further study into concepts of laboratory methods using systems biology.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Klappentext
In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cytotoxic T-Cells: Methods and Protocols seeks to aid scientists in the further study into concepts of laboratory methods using systems biology.

Inhalt
Negative And Positive Separation Techniques For The Isolation Of Antigen-Specific Cd8 + T Cells From Blood And Tumor Tissue.- In Vitro\Ex Vivo Generation Of Cytotoxic T Lymphocytes.- Phenotypic And Functional Characterization Of Cytotoxic T Lymphocytes By Flow Cytometry.- Intracellular Signalling Of Ctls.- Monitoring Antigen-Specific T Cell Responses Using Real-Time Pcr.- Ctl Elispot Assay.- Methods Of Purification Of Ctl-Derived Exosomes.- Characterization Of Ctl By Microscopy.- Intravital Imaging Of Cytotoxic T Lymphocytes.- The Microarray-Based Approach For The Analysis Of The Transcriptome.- Mirnome Analysis Using Real Time Pcr.- Proteomic Approaches By Seldi And Maldi-Tof/Ms For Ctl Analysis.- Two-Dimensional Gel Electrophoresis Approach For Ctl Phosphoproteome Analysis.- Targeting The Jak/Stat Pathway In Cytotoxic T Lymphocytes (Ctl) By Next Generation Sequencing (Ngs).- Experimental Model For Studying The Involvement Of Regulatory Cytotoxic T Cells In Bone Resorption.- Ctl And Transplantation: Tissue In Vivo Characterization.