The fourth edition provides new and updated chapters that focus on experimental models, useful for investigating various aspects of cytoskeleton structure and function. Chapters detail protocols on animal models, plant models, protist models, fungal models, live and fixed-cell imaging, dynamics of cytoskeleton components, cell and organelle motility, genetics, and proteomics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Cytoskeleton: Methods and Protocols, Fourth Edition aims to provide an updated version to help researchers expand their understanding of cytoskeleton structure and function.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible result Contains key notes and implementation advice from the experts

Inhalt
Super Resolution Imaging of the Actin Cytoskeleton in Living Cells Using TIRF-SIM.- Imaging Cytoskeleton Components by Electron Microscopy.- Imaging the Actin Cytoskeleton in Live Budding Yeast Cells.- Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells.- Imaging of actin cytoskeletal integrity during aging in C. elegans.- Imaging the Cytoskeleton in Living Plant Roots.- Imaging of Actin Cytoskeleton in the Nematode Caenorhabditis elegans.- Imaging of the Cytoskeleton using Live and Fixed Drosophila Tissue Culture Cells.- Influencing the Actin-Dynamics in Plant Cells by Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins.- Analysis of Morphogenesis and Flagellar Assembly During Spermatogenesis in Planarian Flatworms.- An In Vitro Model System to Test Mechano-Microbiological Interactions Between Bacteria and Host Cells.- Purification of Cytoskeletal Proteins by Fast Protein Liquid Chromatography (FPLC)Using an ÄKTA Start System.- The Cilioprotist Cytoskeleton, a Model for Understanding How Cell Architecture and Pattern Are Specified: Recent Discoveries from Ciliates and Comparable Model Systems.- 3D and 4D Tumorigenesis Model for the Quantitative Analysis of Cancer Cell Behavior and Screening for Anti-Cancer Drugs.- Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells.- Chemotaxis: Under Agarose Assay.- Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila.- Using a Hand-Held Gene Gun for Genetic Transformation of Tetrahymena thermophila.- Proteomic Tools for Analysis of Cytoskeleton Proteins.