John Sinclair and a panel of expert investigators present a comprehensive collection of cellular and molecular techniques for the analysis of cytomegalovirus (CMV) biology and its pathogenetic mechanisms. The methods-all described in step-by-step detail with ready reproducibility in mind-range from basic virus culture to complex molecular analysis of CMV structure and function. Included are methods for CMV detection using both immunological and biological techniques, methods for analyzing fundamental aspects of the CMV infection cycle, and methods for analyzing T cell response to cytomegalovirus infection in the human host. Comprehensive and state-of-the-art, Cytomegalovirus Protocols provides investigators with a collection of the key methods that are illuminating not only the basic biology of this complex and intriguing human herpesvirus, but also its significant role in human infectious diseases and their emergent therapies.

Klappentext

In Cytomegalovirus Protocols (CMV), John Sinclair and a panel of expert investigators present a comprehensive collection of cellular and molecular techniques for the analysis of cytomegalovirus biology and its pathogenetic mechanisms. The methods-all described in step-by-step detail with ready reproducibility in mind-range from basic virus culture to complex molecular analysis of CMV structure and function. Included are methods for CMV detection using both immunological and biological techniques, methods for analyzing fundamental aspects of the CMV infection cycle, and methods for analyzing T cell response to cytomegalovirus infection in the human host.
Comprehensive and state-of-the-art, Cytomegalovirus Protocols provides investigators with a powerful collection of the key methods that are illuminating not only the basic biology of this complex and intriguing human herpesvirus, but also its significant role in the pathogenesis of human infectious diseases as well as their emergent therapies.

Zusammenfassung
Included are methods for CMV detection using both immunological and biological techniques, methods for analyzing fundamental aspects of the CMV infection cycle, and methods for analyzing T cell response to cytomegalovirus infection in the human host.

Inhalt
Immunological Methods for the Detection of Human Cytomegalovirus.- Identification of Immunoreactive Viral Proteins.- Analysis of Human Cytomegalovirus Using the Polymerase Chain Reaction.- Analysis of Cytomegalovirus Ligands, Receptors, and the Entry Pathway.- Analysis of Cytomegalovirus Gene Expression by Transfection.- Analysis of Protein-Protein Interactions During Cytomegalovirus Infection.- Analysis of Protein-DNA Interactions During Cytomegalovirus Infection.- Analysis of Cytotoxic T-Cell Responses to Human Cytomegalovirus Infection.- Methods in Anti-HCMV Research.- Construction of Recombinant Human Cytomegalovirus.