Includes information on a new model eukaryote for cell and organelle biology, Cyanidioschyzon merolae

Presents molecular cell biological and cytological techniques including synchronization, microscopy and genetic engineering A definitive guide for both beginners and professionals in studying Cyanidioschyzon merolae

Includes information on a new model eukaryote for cell and organelle biology, Cyanidioschyzon merolae Presents molecular cell biological and cytological techniques including synchronization, microscopy and genetic engineering A definitive guide for both beginners and professionals in studying Cyanidioschyzon merolae

Autorentext
Tsuneyoshi Kuroiwa
Biological Imaging Center, Japan Women's University
Tokyo, Japan

Shinya Miyagishima
Department of Cell Genetics, National Institute of Genetics
Shizuoka, Japan

Sachihiro Matsunaga
Dept of Applied Biological Science, Tokyo University of Science
Chiba, Japan

Naoki Sato
Department of Life Sciences, University of Tokyo
Tokyo, Japan

Hisayoshi Nozaki
Department of Biological Science, University of Tokyo
Tokyo, Japan

Kan Tanaka
Chemical Resources Laboratory, Tokyo Institute of Technology
Kanagawa, Japan

Osami Misumi
Dept of Biological Science and Chemistry, Yamaguchi University
Yamaguchi, Japan

Inhalt

Preface.- Part I. Birth, evolution and structure of primary eukaryote.- Chapter 1. The Genus Cyanidiales: evolution and habitat.- Chapter 2. Cellular structure of Cyanidioschyzon merolae : minimum set of organelles.- Part II. Synchronous culture of Cyanidioschyzon merolae and isolation of organelles.- Chapter 3. Cultivation, observation and application of conventional experimental procedures.- Chapter 4. Isolation of (dividing) organelles.- Part III. Genome and post-genomic analyses of Cyanidioschyzon merolae .- Chapter 5. 100% complete nuclear and organellar genome sequences.- Chapter 6. Omics analyses of Cyanidioschyzon merolae based on complete genome information.- Chapter 7. Procedures for transformation and their application.- Part IV. Techniques for Morphological and Structural Biology .- Chapter 8. Basic techniques for fluorescence and electron microscopy.- Chapter 9. Cytological analyses by advanced electron mic roscopy.- Chapter 10. Structural analyses by cryogenic coherent X-ray diffraction imagingPart V. Proliferation of Eukaryotic Cell.- Chapter 11. Cell cycle and organelle division cycle.- Chapter 12. Synchronization of cell cycle by light and circadian rhythms.- Chapter13. Control of cell nuclear DNA replication by chloroplast and mitochondrion.- Chapter 14. Double-membrane-bounded organelles.- Chapter 15. Single-membrane-bounded organelles.- Chapter 16. Control of organelle division.- Part VI. Metabolism.- Chapter 17. Photosynthesis.- Chapter 18. Nitrogen metabolism.- Chapter 19. Carbon metabolism.- Part VII. Application.- Chapter 20. Application of the tolerance to extreme environment to other organisms.- Chapter 21. Metabolic engineering.- Appendix.- Index. <p